| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Section of Infectious Diseases, Department of Pediatrics, Houston, Texas 77030
2 Section of Molecular Pathobiology, Department of Pathology, Houston, Texas 77030
5 Baylor College of Medicine, and Clinical Microbiology Laboratory and Molecular Diagnostics Laboratory; The Methodist Hospital, Houston, Texas 77030
3 Infectious Disease Section, Durham Veterans Affairs Medical Center, Durham, North Carolina 27705
4 Division of Infectious Diseases and International Health, Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710
* Corresponding author. Mailing address: Department of Pathology, Baylor College of Medicien, One Baylor Plaza, Houston, TX 77030. Phone: (713) 798-4198. Fax: (713) 798-4595. E-mail: jmusser{at}path.bcm.tmc.edu.
ABSTRACT
To develop a strategy for rapid species assignment and strain differentiation of Mycobacterium avium complex (MAC) organisms, the sequence of a 360-bp region of the gene (hsp65) encoding a 65-kDa heat shock protein was determined for 56 isolates, including 21 patient isolates and 35 reference strains. Eleven hsp65 alleles were identified, and there was no sharing of alleles between strains classified as M. avium and Mycobacterium intracellulare based on serovar and species-specific DNA hybridization probes. Phylogenetic analysis showed that 30 strains had one of two hsp65 alleles which were found in known M. avium organisms, 23 strains had one of six alleles allied with known M. intracellulare organisms, and three MAC isolates had one of three hsp65 alleles that differed substantially from the consensus M. avium and M. intracellulare hsp65 sequences. Estimates of strain relationships based on the sequences of hsp65 and the 16S-23S ribosomal DNA internal transcribed spacer were similar. Automated DNA sequencing of a 360-bp region of the hsp65 gene from MAC organisms provides a rapid and unambiguous marker system for strain differentiation and permits specific assignment of these acid-fast organisms for diagnostic purposes.
present address: Department of Veterinary Pathobiology, University of Minnesota, St. Paul, MN 55108. This article has been cited by other articles:
|
|
 |
|
  N. Mackenzie, D. C. Alexander, C. Y. Turenne, M. A. Behr, and J. M. De Buck Genomic Comparison of PE and PPE Genes in the Mycobacterium avium Complex J. Clin. Microbiol., April 1, 2009; 47(4): 1002 - 1011. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Ben Salah, T. Adekambi, D. Raoult, and M. Drancourt rpoB sequence-based identification of Mycobacterium avium complex species Microbiology, December 1, 2008; 154(12): 3715 - 3723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Stout, G. W. Hopkins, J. R. McDonald, A. Quinn, C. D. Hamilton, L. B. Reller, and R. Frothingham Association between 16S-23S Internal Transcribed Spacer Sequence Groups of Mycobacterium avium Complex and Pulmonary Disease J. Clin. Microbiol., August 1, 2008; 46(8): 2790 - 2793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Richter, S. Rusch-Gerdes, and D. Hillemann Evaluation of the GenoType Mycobacterium Assay for Identification of Mycobacterial Species from Cultures. J. Clin. Microbiol., May 1, 2006; 44(5): 1769 - 1775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Turenne, M. Semret, D. V. Cousins, D. M. Collins, and M. A. Behr Sequencing of hsp65 Distinguishes among Subsets of the Mycobacterium avium Complex J. Clin. Microbiol., February 1, 2006; 44(2): 433 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, M. A. Yakrus, E. A. Graviss, N. Williams-Bouyer, C. Turenne, A. Kabani, and R. J. Wallace Jr. Pulsed-Field Gel Electrophoresis Study of Mycobacterium abscessus Isolates Previously Affected by DNA Degradation J. Clin. Microbiol., December 1, 2004; 42(12): 5582 - 5587. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. McNabb, D. Eisler, K. Adie, M. Amos, M. Rodrigues, G. Stephens, W. A. Black, and J. Isaac-Renton Assessment of Partial Sequencing of the 65-Kilodalton Heat Shock Protein Gene (hsp65) for Routine Identification of Mycobacterium Species Isolated from Clinical Sources J. Clin. Microbiol., July 1, 2004; 42(7): 3000 - 3011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Krzywinska, J. Krzywinski, and J. S. Schorey Phylogeny of Mycobacterium avium strains inferred from glycopeptidolipid biosynthesis pathway genes Microbiology, June 1, 2004; 150(6): 1699 - 1706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Smole, F. McAleese, J. Ngampasutadol, C. F. von Reyn, and R. D. Arbeit Clinical and Epidemiological Correlates of Genotypes within the Mycobacterium avium Complex Defined by Restriction and Sequence Analysis of hsp65 J. Clin. Microbiol., September 1, 2002; 40(9): 3374 - 3380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Menendez, E. Palenque, M. C. Navarro, M. C. Nunez, M. J. Rebollo, and M. J. Garcia Characterization of a Mycobacterium intracellulare Variant Strain by Molecular Techniques J. Clin. Microbiol., December 1, 2001; 39(12): 4241 - 4246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Hedegaard, H. Okkels, B. Bruun, M. Kilian, K. K. Mortensen, and N. Norskov-Lauritsen Phylogeny of the genus Haemophilus as determined by comparison of partial infB sequences Microbiology, September 1, 2001; 147(9): 2599 - 2609. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Oliveira, M. P. Sircili, S. Y. M. Ueki, M. A. S. Telles, B. Schnabel, M. R. S. Briones, and S. C. Leão PCR-Restriction Enzyme Analysis of a Bone Marrow Isolate from a Human Immunodeficiency Virus-Positive Patient Discloses Polyclonal Infection with Two Mycobacterium avium Strains J. Clin. Microbiol., December 1, 2000; 38(12): 4643 - 4645. [Abstract] [Full Text]
|
|
|
|
|
|
M. L. Beggs, R. Stevanova, and K. D. Eisenach Species Identification of Mycobacterium avium Complex Isolates by a Variety of Molecular Techniques J. Clin. Microbiol., February 1, 2000; 38(2): 508 - 512. [Abstract] [Full Text]
|
|
|
|
|
|
H. Kasai, T. Ezaki, and S. Harayama Differentiation of Phylogenetically Related Slowly Growing Mycobacteria by Their gyrB Sequences J. Clin. Microbiol., January 1, 2000; 38(1): 301 - 308. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. Velaz-Faircloth, A. J. Cobb, A. L. Horstman, S. C. Henry, and R. Frothingham Protection against Mycobacterium avium by DNA Vaccines Expressing Mycobacterial Antigens as Fusion Proteins with Green Fluorescent Protein Infect. Immun., August 1, 1999; 67(8): 4243 - 4250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Leão, M. R. S. Briones, M. P. Sircili, S. C. Balian, N. Mores, and J. S. Ferreira-Neto Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR-Restriction Enzyme Analysis J. Clin. Microbiol., August 1, 1999; 37(8): 2592 - 2597. [Abstract] [Full Text]
|
|
|
|
|
|
H. Ringuet, C. Akoua-Koffi, S. Honore, A. Varnerot, V. Vincent, P. Berche, J. L. Gaillard, and C. Pierre-Audigier hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria J. Clin. Microbiol., March 1, 1999; 37(3): 852 - 857. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
