HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by AVGUSTIN, G. Articles by FLINT, H. J. Search for Related Content PubMed Articles by AVGUSTIN, G. Articles by FLINT, H. J. Agricola Articles by AVGUSTIN, G. Articles by FLINT, H. J.

Phenotypic Diversity among Ruminal Isolates of Prevotella ruminicola: Proposal of Prevotella brevis sp. nov., Prevotella bryantii sp. nov., and Prevotella albensis sp. nov. and Redefinition of Prevotella ruminicola

GORAZD AVGUTIN¹, R. JOHN WALLACE² and HARRY J. FLINT^2,*

¹ Zootechnical Department, Biotechnical Faculty, University of Ljubljana, 1230 Domzale, Slovenia
² Rowett Research Institute, Bucksburn, Aberdeen AB21 9SB, United Kingdom

^* Corresponding author. Mailing address: Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom. Phone: (0) 1224 716651. Fax: (0) 1224 716687. E-mail: h.flint{at}rri.sari.ac.uk.

ABSTRACT

Selected phenotypic characteristics of isolates of Prevotella ruminicola (formerly Bacteroides ruminicola) were studied in order to establish whether the characteristics of genotypic strain groups established previously on the basis of 16S ribosomal DNA sequences differed systematically. Among strains formerly considered P. ruminicola subsp. brevis, strains related to strain GA33^T (T = type strain) typically failed to produce carboxymethyl cellulase (CMCase) activity detectable by plate assays and failed to ferment xylose, while strains related to strain B₁4^T produced abundant CMCase and fermented xylose. We propose that strains related to GA33^T, which have DNA G+C contents between 45 and 51 mol%, should be assigned to a new species, Prevotella brevis, and that strains related to B₁4^T, which have DNA G+C contents between 39 and 43 mol%, should be assigned to another new species, Prevotella bryantii. Most of the isolates formerly classified as P. ruminicola subsp. ruminicola strains produced CMCase and had DNA G+C contents between 45 and 51 mol%, and we propose that these organisms should be placed in the redefined species P. ruminicola. A small group of isolates that have lower G+C contents are assigned to another new species, Prevotella albensis. Most P. brevis and P. bryantii strains produced abundant extracellular DNase activity. Proteinase activities (as determined by [¹⁴C] casein hydrolysis) varied widely between strains, and P. brevis strains exhibited the highest mean activity. All strains produced dipeptidyl peptidase activity, but the relative activities against different peptide substrates exhibited by P. bryantii, P. albensis, and P. brevis differed systematically. The phenotypic differences among the newly defined species suggest that they may occupy distinct niches within the rumen ecosystem.

This article has been cited by other articles:

				C. Alauzet, F. Mory, J.-P. Carlier, H. Marchandin, E. Jumas-Bilak, and A. Lozniewski Prevotella nanceiensis sp. nov., isolated from human clinical samples Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2216 - 2220. [Abstract] [Full Text] [PDF]

				A. Ueki, H. Akasaka, A. Satoh, D. Suzuki, and K. Ueki Prevotella paludivivens sp. nov., a novel strictly anaerobic, Gram-negative, hemicellulose-decomposing bacterium isolated from plant residue and rice roots in irrigated rice-field soil Int J Syst Evol Microbiol, August 1, 2007; 57(8): 1803 - 1809. [Abstract] [Full Text] [PDF]

				T. Accetto and G. Avgustin Studies on Prevotella nuclease using a system for the controlled expression of cloned genes in P. bryantii TC1-1 Microbiology, July 1, 2007; 153(7): 2281 - 2288. [Abstract] [Full Text] [PDF]

				O. O. Glazunova, T. Launay, D. Raoult, and V. Roux Prevotella timonensis sp. nov., isolated from a human breast abscess Int J Syst Evol Microbiol, April 1, 2007; 57(4): 883 - 886. [Abstract] [Full Text] [PDF]

				A. Ueki, H. Akasaka, D. Suzuki, S. Hattori, and K. Ueki Xylanibacter oryzae gen. nov., sp. nov., a novel strictly anaerobic, Gram-negative, xylanolytic bacterium isolated from rice-plant residue in flooded rice-field soil in Japan. Int J Syst Evol Microbiol, September 1, 2006; 56(Pt 9): 2215 - 2221. [Abstract] [Full Text] [PDF]

				D. Schmitt-Wagner, M. W. Friedrich, B. Wagner, and A. Brune Phylogenetic Diversity, Abundance, and Axial Distribution of Bacteria in the Intestinal Tract of Two Soil-Feeding Termites (Cubitermes spp.) Appl. Envir. Microbiol., October 1, 2003; 69(10): 6007 - 6017. [Abstract] [Full Text] [PDF]

				N. D. Walker, N. R. McEwan, and R. J. Wallace Cloning and functional expression of dipeptidyl peptidase IV from the ruminal bacterium Prevotella albensis M384T Microbiology, August 1, 2003; 149(8): 2227 - 2234. [Abstract] [Full Text] [PDF]

				J. Kopecny, M. Zorec, J. Mrazek, Y. Kobayashi, and R. Marinsek-Logar Butyrivibrio hungatei sp. nov. and Pseudobutyrivibrio xylanivorans sp. nov., butyrate-producing bacteria from the rumen Int J Syst Evol Microbiol, January 1, 2003; 53(1): 201 - 209. [Abstract] [Full Text] [PDF]

				J. B. Russell and J. L. Rychlik Factors That Alter Rumen Microbial Ecology Science, May 11, 2001; 292(5519): 1119 - 1122. [Abstract] [Full Text]

				M. W. Fields and J. B. Russell The glucomannokinase of Prevotella bryantii B14 and its potential role in regulating {beta}-glucanase expression Microbiology, April 1, 2001; 147(4): 1035 - 1043. [Abstract] [Full Text]

				A. Barcenilla, S. E. Pryde, J. C. Martin, S. H. Duncan, C. S. Stewart, C. Henderson, and H. J. Flint Phylogenetic Relationships of Butyrate-Producing Bacteria from the Human Gut Appl. Envir. Microbiol., April 1, 2000; 66(4): 1654 - 1661. [Abstract] [Full Text]

				S. E. Pryde, A. J. Richardson, C. S. Stewart, and H. J. Flint Molecular Analysis of the Microbial Diversity Present in the Colonic Wall, Colonic Lumen, and Cecal Lumen of a Pig Appl. Envir. Microbiol., December 1, 1999; 65(12): 5372 - 5377. [Abstract] [Full Text]

				T. R. Callaway and J. B. Russell Selection of a Highly Monensin-Resistant Prevotella bryantii Subpopulation with Altered Outer Membrane Characteristics Appl. Envir. Microbiol., November 1, 1999; 65(11): 4753 - 4759. [Abstract] [Full Text]

				F. Floret, L. C. Chaudhary, W. C. Ellis, S. El Hassan, N. McKain, C. J. Newbold, and R. J. Wallace Influence of 1-[(E)-2-(2-Methyl-4-Nitrophenyl)Diaz-1-enyl]Pyrrolidine-2-Carboxylic Acid and Diphenyliodonium Chloride on Ruminal Protein Metabolism and Ruminal Microorganisms Appl. Envir. Microbiol., July 1, 1999; 65(7): 3258 - 3260. [Abstract] [Full Text]

				J. Wood, K. P. Scott, C. J. Newbold, and H. J. Flint Estimation of the Relative Abundance of Different Bacteroides and Prevotella Ribotypes in Gut Samples by Restriction Enzyme Profiling of PCR-Amplified 16S rRNA Gene Sequences Appl. Envir. Microbiol., October 1, 1998; 64(10): 3683 - 3689. [Abstract] [Full Text]