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1 Department of Clinical Microbiology, Herlev Hospital, DK-2730 Herlev
5 Department of Clinical Microbiology, Bispebjerg Hospital, DK-2400 Copenhagen, Denmark
2 Emerging Bacterial and Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
4 Respiratory Diseases Branch
6 Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333
3 Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941, Brazil
* Corresponding author. Mailing address:Department of Clinical Microbiology, Århus University Hospital. Nørrebrogade 44, 8000 Århus C, Denmark. Phone: 45 8949 3527. Fax: 45 8949 3540.
ABSTRACT
A number of Arococcus-like organisms were recently recognized as human pathogens. Five Aerococcus-like strains were proposed as members of the new species Aerococcus urinae (with type strain E2 [= NCTC 12142]) on the basis of the results of a 16S rRNA sequence analysis. The intraspecies phenotypic and genetic relatedness of 22 selected A. urinae strains was investigated, and a hitherto unrecognized esculin hydrolysis-positive biotype was identified. A total of 14 of the 15 more common esculin-negative strains exhibited very high DNA relatedness as determined by the hydroxyapatite method (the levels of relatedness were greater than 90% in 55 and 70°C reactions, with 1.5% or less divergence in related sequences). The DNA relatedness among the six esculin-positive strains was more heterogeneous, and two DNA hybridization subgroups were formed. Our results are compatible with the hypothesis that both biotypes are members of the single species A. urinae, which contains two or more genetic subspecies. The putative subspecies have not been formally proposed since they cannot be definitively differentiated. The inclusion of A. urinae in the genus Aerococcus is supported by the results of 16S rRNA sequencing. The rRNA sequence data also is compatible with placing both biotypes in a single species.
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