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Int J Syst Bacteriol 47 (1997), 127-131; DOI 10.1099/00207713-47-1-127
© 1997 Society for General Microbiology
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A Proposal To Reclassify Nocardia pinensis Blackall et al. as Skermania piniformis gen. nov., comb. nov.

JONGSIK CHUN1,2, LINDA L. BLACKALL3, SA-OUK KANG1, YUNG CHIL HAH1 and MICHAEL GOODFELLOW2,*

1 Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University, Seoul 151-742, Republic of Korea
2 Department of Microbiology, The Medical School, Framlington Place, Newcastle upon Tyne, NE2 4HH, United Kingdom
3 Center for Bacterial Diversity and Identification. Department of Microbiology, The University of Queensland, Brisbane, 4072, Queensland, Australia

* Corresponding author. Fax: 44–91–222–7736.

ABSTRACT

The type strain of Nocardia pinensis was the subject of chemotaxonomic and 16S ribosomal DNA sequencing studies. The resultant nucleotide sequence was aligned with the sequences of representatives of the genera Corynebacterium, Dietzia. Gordona, Mycobacterium, Nocardia, Rhodococcus, and Tsukamurella, and phylogenetic trees were generated by using the Fitch-Margoliash, maximum-parsimony, maximum-likelihood, and neighbor-joining methods. It was evident from the phylogenetic analyses that N. pinensis represents a distinct phyletic line that is most closely associated with the Gordona clade. This genealogical evidence, together with chemotaxonomic and phenotypic data derived from this and previous studies, indicates that N. pinensis merits generic status within the family Nocardiaceae. Therefore, we propose that N. pinensis Blackall et al. 1989 be reclassified as Skermania piniformis gen. nov., comb. nov. The type strain of Skermania piniformis cleaved an array of conjugated substrates based on the fluorophores 7-amino-4-methylcoumarin and 4-methylumbelliferone.




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