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Spiroplasma corruscae sp. nov., from a Firefly Beetle (Coleoptera: Lampyridae) and Tabanid Flies (Diptera: Tabanidae)

K. J. HACKETT^1,*, R. F. WHITCOMB¹, F. E. FRENCH², J. G. TULLY³, G. E. GASPARICH¹, D. L. ROSE³, P. CARLE⁴, J. M. BOVE⁴, R. B. HENEGAR¹, T. B. CLARK^1,, M. KONAI¹, E. A. CLARK¹ and D. L. WILLIAMSON⁵

¹Insect Biocontrol Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705
²Department of Biology, Georgia Southern College, Statesboro, Georgia 30460
³Mycoplasma Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Frederick Cancer Research Facility, Frederick, Maryland 21702
⁴Laboratoire de Biologie Cellulii et Moleculaire, Institut Nationale de Recherche Agronomique, 33883 Villenave d'Ornon, France
⁵Department of Anatomical Sciences, State University of New York, Stony Brook, New York 11794

^* Corresponding author. Mailing address: Building 011 A, Room 214, BARC-West, Beltsville, MD 20705. Phone: (301) 504-6325. Fax: (301) 504-5104.

ABSTRACT

Spiroplasma strain EC-1^T (T = type strain), which was isolated from the gut of a lampyrid beetle (Ellychnia corrusca) in Maryland, was serologically distinct from other spiroplasma species and groups. Similar strains were obtained from other E. corrusca specimens, and, later, numerous isolates of similar or partially related strains were obtained from several species of tabanid flies. Cells of strain EC-1^T were helical, motile filaments that were bound by a single cytoplasmic membrane, and there was no evidence of a cell wall. The cells were filterable through 220-nm-pore-size membrane filters but not through 100-nm-pore-size membrane filters. The organism was absolutely resistant to penicillin (1,000 U/ml) and required sterol for growth. Strain EC-1^T grew well in MID and SP-4 liquid media and could be cultivated in the Edward formulation of conventional mycoplasma medium and in 1% serum fraction medium. Optimal growth occurred at 32°C (doubling time, 1.5 h). Strain EC-1^T multiplied at 10 to 41°C, but not at 5 or 43°C. This organism produced acid from glucose, but did not hydrolyze arginine or utilize urea. The guanine-plus-cytosine content of the DNA was determined to be 26.3 mol% by the melting temperature method and 27.0 mol% by the buoyant density method. As a result of our studies, strain EC-1 (= ATCC 43212) is designated the type strain of a new species, Spiroplasma corruscae.

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