| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-02, Japan
2Department of Parasitology, Asahikawa Medical College, Asahikawa, Hokkaido 078, Japan
* Corresponding author. Mailing address: Laboratory of Molecular Microbiology, Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Sanzo 985, Gakucncho 1, Fukuyama, Hiroshima 729-02, Japan. Phone: 81 849 36 2111. Fax: 81 849 36 2024. Electronic mail address: mfukunag{at}supernig.nig.ac.jp.
ABSTRACT
We determined almost complete flagellin gene sequences of various Borrelia species and aligned them with previously published sequences. A neighbor-joining phylogenetic analysis showed that the genus Borrelia was divided into the following three major clusters: New World relapsing fever borreliae (Borrelia turicatae, Borrelia parkeri and Borrelia hermsii), Old World relapsing fever borreliae (Borrelia crocidurae, Borrelia duttonii, and Borrelia hispanica), and Lyme disease borreliae (Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii). Agents of animal spirochetosis (Borrelia coriaceae and Borrelia anserina) and species of unknown pathogenicity (Borrelia miyamotoi and Borrelia lonestari) were related to relapsing fever borreliae. Although the Lyme disease borreliae, two related species (Borrelia japonica and Borrelia andersonii), and some newly described genomic groups (groups PotiB2, VS116, DN127, Hk501, and Ya501) were closely related to each other, each taxon formed an independent branch on the phylogenetic tree. The data obtained in this study indicate that the flagellin genes are useful in Borrelia taxonomy. To distinguish the Lyme disease borreliae from related organisms easily, we designed an oligonucleotide primer set for the flagellin gene and performed a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. The primer set amplified an approximately 580-bp DNA fragment that included species-specific restriction sites, and Hapll, Hhal, CelII, HincII, or Ddel digestion of the product resulted in distinctively different PCR-RFLP patterns. The PCR-RFLP typing method which we developed should facilitate rapid identification of Lyme disease borreliae and related organisms obtained from biological and clinical specimens.
This article has been cited by other articles:
|
|
 |
|
  J. M. Anderson and D. E. Norris Genetic Diversity of Borrelia burgdorferi Sensu Stricto in Peromyscus leucopus, the Primary Reservoir of Lyme Disease in a Region of Endemicity in Southern Maryland Appl. Envir. Microbiol., August 1, 2006; 72(8): 5331 - 5341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Richter and F.-R. Matuschka Perpetuation of the Lyme Disease Spirochete Borrelia lusitaniae by Lizards. Appl. Envir. Microbiol., July 1, 2006; 72(7): 4627 - 4632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. G. Schwan, S. J. Raffel, M. E. Schrumpf, P. F. Policastro, J. A. Rawlings, R. S. Lane, E. B. Breitschwerdt, and S. F. Porcella Phylogenetic Analysis of the Spirochetes Borrelia parkeri and Borrelia turicatae and the Potential for Tick-Borne Relapsing Fever in Florida J. Clin. Microbiol., August 1, 2005; 43(8): 3851 - 3859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Clark, A. Hendricks, and D. Burge Molecular Identification and Analysis of Borrelia burgdorferi Sensu Lato in Lizards in the Southeastern United States Appl. Envir. Microbiol., May 1, 2005; 71(5): 2616 - 2625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Gil, M. Barral, R. Escudero, A. L. Garcia-Perez, and P. Anda Identification of a New Borrelia Species among Small Mammals in Areas of Northern Spain Where Lyme Disease Is Endemic Appl. Envir. Microbiol., March 1, 2005; 71(3): 1336 - 1345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Richter, D. B. Schlee, R. Allgower, and F.-R. Matuschka Relationships of a Novel Lyme Disease Spirochete, Borrelia spielmani sp. nov., with Its Hosts in Central Europe Appl. Envir. Microbiol., November 1, 2004; 70(11): 6414 - 6419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Clark Borrelia Species in Host-Seeking Ticks and Small Mammals in Northern Florida J. Clin. Microbiol., November 1, 2004; 42(11): 5076 - 5086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Bacon, M. A. Pilgard, B. J. B. Johnson, S. J. Raffel, and T. G. Schwan Glycerophosphodiester Phosphodiesterase Gene (glpQ) of Borrelia lonestari Identified as a Target for Differentiating Borrelia Species Associated with Hard Ticks (Acari:Ixodidae) J. Clin. Microbiol., May 1, 2004; 42(5): 2326 - 2328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Masuzawa, N. Hashimoto, M. Kudeken, T. Kadosaka, M. Nakamura, H. Kawabata, N. Koizumi, and Y. Imai New genomospecies related to Borrelia valaisiana, isolated from mammals in Okinawa archipelago, Japan J. Med. Microbiol., May 1, 2004; 53(5): 421 - 426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Derdakova, L. Beati, B. Pet'ko, M. Stanko, and D. Fish Genetic Variability within Borrelia burgdorferi Sensu Lato Genospecies Established by PCR-Single-Strand Conformation Polymorphism Analysis of the rrfA-rrlB Intergenic Spacer in Ixodes ricinus Ticks from the Czech Republic Appl. Envir. Microbiol., January 1, 2003; 69(1): 509 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Fraenkel, U. Garpmo, and J. Berglund Determination of Novel Borrelia Genospecies in Swedish Ixodes ricinus Ticks J. Clin. Microbiol., September 1, 2002; 40(9): 3308 - 3312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. DEMIRKAN, S.D. CARTER, C. WINSTANLEY, K.D. BRUCE, N.M. McNAIR, M. WOODSIDE, and C.A. HART Isolation and characterisation of a novel spirochaete from severe virulent ovine foot rot J. Med. Microbiol., December 1, 2001; 50(12): 1061 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Carter, M. D. Ravyn, Y. Xu, and R. C. Johnson Molecular Typing of the Etiologic Agent of Human Granulocytic Ehrlichiosis J. Clin. Microbiol., September 1, 2001; 39(9): 3398 - 3401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Lee, B.-J. Kim, J.-H. Kim, K.-H. Park, S.-J. Kim, and Y.-H. Kook Differentiation of Borrelia burgdorferi Sensu Lato on the Basis of RNA Polymerase Gene (rpoB) Sequences J. Clin. Microbiol., July 1, 2000; 38(7): 2557 - 2562. [Abstract] [Full Text]
|
|
|
|
|
|
M. Hendson and R. S. Lane Genetic Characteristics of Borrelia coriaceae Isolates from the Soft Tick Ornithodoros coriaceus (Acari: Argasidae) J. Clin. Microbiol., July 1, 2000; 38(7): 2678 - 2682. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Wang, A. P. van Dam, I. Schwartz, and J. Dankert Molecular Typing of Borrelia burgdorferi Sensu Lato: Taxonomic, Epidemiological, and Clinical Implications Clin. Microbiol. Rev., October 1, 1999; 12(4): 633 - 653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Postic, N. M. Ras, R. S. Lane, M. Hendson, and G. Baranton Expanded Diversity among Californian Borrelia Isolates and Description of Borrelia bissettii sp. nov. (Formerly Borrelia Group DN127) J. Clin. Microbiol., December 1, 1998; 36(12): 3497 - 3504. [Abstract] [Full Text]
|
|
|
|
|
|
M. Li, T. Masuzawa, N. Takada, F. Ishiguro, H. Fujita, A. Iwaki, H. Wang, J. Wang, M. Kawabata, and Y. Yanagihara Lyme Disease Borrelia Species in Northeastern China Resemble Those Isolated from Far Eastern Russia and Japan Appl. Envir. Microbiol., July 1, 1998; 64(7): 2705 - 2709. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. A. Carlyon, C. Lavoie, S.-Y. Sung, and R. T. Marconi Analysis of the Organization of Multicopy Linear- and Circular-Plasmid-Carried Open Reading Frames in Borrelia burgdorferi Sensu Lato Isolates Infect. Immun., March 1, 1998; 66(3): 1149 - 1158. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
