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Phylogeny of Some Mycoplasmas from Ruminants Based on 16S rRNA Sequences and Definition of a New Cluster within the Hominis Group

¹Department of Biochemistry and Biotechnology, The Royal Institute of Technology, S-100 44 Stockholm
²Department of Bacteriology, National Veterinary Institute, S-750 07 Uppsala, Sweden
³Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, S-750 07 Uppsala, Sweden

^* Corresponding author. Mailing address: Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, P.O. Box 7073, S-750 07 Uppsala, Sweden. Phone: 46 18 67 40 00. Fax: 46 18 30 91 62. Electronic mail address: Kaggen{at}sva.se.

ABSTRACT

Almost complete (>96%) 16S rRNA sequences from nine ruminant mycoplasmas have been determined by solid-phase DNA sequencing. Polymorphisms were found in four of the 16S rRNA sequences, which indicated the existence of two different rRNA operons. Seven polymorphisms were found in Mycoplasma agalactiae, three were found in Mycoplasma bovis, one was found in Mycoplasma alkalescens, and one was found in Mycoplasma bovirhinis. The sequence data were used for construction of phylogenetic trees. All but one of the ruminant mycoplasmas sequenced in this work clustered in the hominis group. A close relationship was found between M. agalactiae and M. bovis, with a 99% nucleotide similarity between their 16S rRNA sequences. They were also found to be members of the Mycoplasma lipophilum cluster of the hominis group. Furthermore, the 16S rRNA comparisons showed that Mycoplasma alkalescens and Mycoplasma canadense are closely related (>98.5%), and these species were found to cluster in the Mycoplasma hominis cluster of the hominis group. Interestingly, M. bovirhinis grouped in a new phylogenetic cluster of the hominis group. The new cluster, which was supported by bootstrap percentage values, signature nucleotide analysis, and higher-order structural elements, was named the Mycoplasma synoviae cluster. Mycoplasma bovoculi, Mycoplasma conjunctivae, and Mycoplasma ovipneumoniae clustered in the Mycoplasma neurolyticum cluster of the hominis group. Mycoplasma alvi clustered with Mycoplasma pirum in the M. pneumoniae cluster of the pneumoniae group.

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