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Int J Syst Bacteriol 46 (1996), 1078-1082; DOI 10.1099/00207713-46-4-1078
© 1996 Society for General Microbiology
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Reassessment of the Phylogenetic Position of the Bacterium Associated with Whipple's Disease and Determination of the 16S-23S Ribosomal Intergenic Spacer Sequence

MATTHIAS MAIWALD1,*, HANS-JÜRGEN DITTON1, AXEL von HERBAY2, FREDERICK A. RAINEY3 and ERKO STACKEBRANDT3

1Hygiene-Institut der Universität, Abteilung Hygiene und Medizinische Mikrobiologie, 69120 Heidelberg
2Pathologisches Institut der Universität, 69120 Heidelberg, Germany
3Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, 38124 Braunschweig, Germany

* Corresponding author. Mailing address: Hygiene-Institut der IJniversitat Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany. Phone: 49-6221-567815. Fax: 49-6221-564343. Electronic mail address: un69mm{at}genius.embnet.dkfz-heidelberg.de.

ABSTRACT

Whipple's disease is a rare chronic illness associated with an unculturable bacterium that is constantly present in affected tissues. This bacterium was previously characterized at the molecular level by PCR and sequencing of the 16S rRNA gene. On the basis of 1,321 nucleotides of the sequence of its gene coding for 16S rRNA (16S rDNA), a phylogenetic relationship to the actinomycetes was established. In this study, we determined an almost complete 16S rDNA sequence (1,495 nucleotides), the 16S-23S ribosomal intergenic spacer sequence, and 200 nucleotides of the 23S rRNA gene. The 16S rDNA sequence was compared with the large number of actinomycete sequences that have been added to the database since the original study. Phylogenetic analysis revealed a branching position as the deepest branch of the cluster comprising the actinomycetes with group B peptidoglycan between this group and the family Cellulomonadaceae. This provides additional information on the phylogenetic position of this bacterium and some clues as to its characteristics. The spacer region between the 16S and 23S rRNA genes is 294 nucleotides long and does not contain tRNA genes. As has been shown in other instances, the increased variability of the ribosomal intergenic spacer compared with the 108 rRNA gene makes it a potential target for use in the differentiation of strains of the bacterium associated with Whipple's disease.




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