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Two New Leptospiral Serovars in the Hebdomadis Serogroup Isolated from Zimbabwe Cattle

¹Department of Biological Sciences. University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe
²N. H. Swellengrebel Laboratory of Tropical Hygiene. The Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands
³Unité de Bactériologie, Moléculaire et Médicale, Institut Pasteur, 75724 Paris Cedex 15, France
⁴Leptospirosis and Mycobacteriosis Research Service. U.S. Department of Agriculture, Ames, Iowa 50010

^* Corresponding author. Mailing address: Department of Biological Science, University of Zimbabwe, MP 167, Mount Pleasant, Harare, Zimbabwe. Phone: 263-4-303211. Fax: 263-4-333407. Electronic mail address: feresu{at}zimbix.uz.zw.

ABSTRACT

Four strains belonging to the genus Leptospira serogroup Hebdomadis were isolated from Zimbabwe cattle at slaughter. These isolates were subjected to cross-agglutinin absorption tests and to restriction fragment length polymorphism and pulsed-field gel electrophoresis analyses of their genomic DNAs. One of these strains repreents a new serovar, for which the name mhou is proposed; strain SBF 40 is the reference strain of this serovar. The other three strains belong to a second new serovar, for which the name marondera is proposed; the reference strain of this serovar is strain SBF 5. The three strains of serovar marondera could be differentiated by their restriction fragment polymorphism and pulsed-field gel electrophoretic patterns.