| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Microbiology, Wageningen Agricultural University, Wageningen, The Netherlands
2 Department of Applied Chemistry and Microbiology, Viikki, University of Helsinki, Helsinki, Finland
3 Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany
* Corresponding author. Mailing address: Department of Microbiology, Wageningen Agricultural University, Hesselink van Suchtelenweg, 4, NL-6703 CT Wageningen, The Netherlands. Phone: 31 (0) 317 4 84099. Fax: 31 (0) 317 4 83829. Electronic mail address: maria.briglia{at}algemeen.micr.wau.nl.
ABSTRACT
A bacterial strain that was able to mineralize 2,4,6-trichlorophenol was isolated from a chlorophenol-fed percolator and was identified as a member of the genus Rhodococcus on the basis of chemotaxonomic characteristics and 16S RNA phylogenetic inference data. This organism (strain MBS1T [T = type strain]) exhibited a typical irregular rod-coccus cycle, and the cells had fimbria-like structures on their surfaces. The diagnostic cell wall amino acid was meso-diaminopimelic acid, and the sugars were arabinose and galactose; the mycolic acids contained 46 to 54 carbon atoms. The main menaquinone was MK-8(H2), and MK-9(H2) was a minor component. The cellular phospholipids were phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylglycerol, and diphosphatidylglycerol. Tuberculostearic acid was present. The whole-cell fatty acids were straight-chain acids with 14 to 18 C atoms. The G + C content of the DNA was 67.4 mol%. This organism grew on sucrose, pyruvate, and 2,4,6-trichlorophenol, and it oxidized a large number of carbon compounds, including catechol, 3-hydroxyphenylacetic acid, and phenol. It also exhibited β-galactosidase, urease, and 2-acetyl-lactate decarboxylase activities. On a phylogenetic tree that was based on 16S ribosomal DNA gene sequences strain MBS1T was found among the rhodococci on an independent branch. On the basis of the chemotaxonomic and phenotypic characteristics of strain MBS1T and its phylogenetic position we suggest that this bacterium should be placed in a new species, Rhodococcus percolatus; the specific epithet was chosen because the organism was isolated by using an enriched percolator. The type strain is strain MBS1.
This article has been cited by other articles:
|
|
 |
|
  M. A. Sanchez and B. Gonzalez Genetic Characterization of 2,4,6-Trichlorophenol Degradation in Cupriavidus necator JMP134 Appl. Envir. Microbiol., May 1, 2007; 73(9): 2769 - 2776. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ghosh, D. Paul, D. Prakash, S. Mayilraj, and R. K. Jain Rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. Int J Syst Evol Microbiol, August 1, 2006; 56(Pt 8): 1965 - 1969. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Garcia, V. Gallego, A. Ventosa, and E. Mellado Thalassobacillus devorans gen. nov., sp. nov., a moderately halophilic, phenol-degrading, Gram-positive bacterium Int J Syst Evol Microbiol, September 1, 2005; 55(5): 1789 - 1795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Yassin Rhodococcus triatomae sp. nov., isolated from a blood-sucking bug Int J Syst Evol Microbiol, July 1, 2005; 55(4): 1575 - 1579. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-Q. Zhang, W.-J. Li, R. M. Kroppenstedt, C.-J. Kim, G.-Z. Chen, D.-J. Park, L.-H. Xu, and C.-L. Jiang Rhodococcus yunnanensis sp. nov., a mesophilic actinobacterium isolated from forest soil Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1133 - 1137. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Li, Y. Kawamura, N. Fujiwara, T. Naka, H. Liu, X. Huang, K. Kobayashi, and T. Ezaki Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir Int J Syst Evol Microbiol, May 1, 2004; 54(3): 827 - 835. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Matus, M. A. Sanchez, M. Martinez, and B. Gonzalez Efficient Degradation of 2,4,6-Trichlorophenol Requires a Set of Catabolic Genes Related to tcp Genes from Ralstonia eutropha JMP134(pJP4) Appl. Envir. Microbiol., December 1, 2003; 69(12): 7108 - 7115. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. Louie, C. M. Webster, and L. Xun Genetic and Biochemical Characterization of a 2,4,6-Trichlorophenol Degradation Pathway in Ralstonia eutropha JMP134 J. Bacteriol., July 1, 2002; 184(13): 3492 - 3500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Koike, K. Ara, S. Adachi, H. Takigawa, H. Mori, S. Inoue, Y. Kimura, and S. Ito Regiospecific Internal Desaturation of Aliphatic Compounds by a Mutant Rhodococcus Strain Appl. Envir. Microbiol., December 1, 1999; 65(12): 5636 - 5638. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
