| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Veterinary Pathology, Veterinary Field Station, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom
3 Centre for Tropical Veterinary Medicine, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, United Kingdom
2 Division of Comparative Medicine, University of Minnesota Medical School, Minneapolis, Minnesota 55455
4 Department of Biology, Institute for Molecular and Cellular Biology, Indiana University, Bloomington, Indiana 47405
* Corresponding author. Phone: 44 31 650 6264. Fax: 44 31 445 5770.
ABSTRACT
A novel obligately intracellular bacterium, ileal symbiont intracellularis, which was obtained from the intestines of pigs with proliferative enteropathy disease, was grown in pure cocultures with tissue cultures of rat cells. An examination of the 16S ribosomal DNA gene sequence revealed that the isolates which we obtained are members of the delta subdivision of the Proteobacteria and that the sequences of these organisms exhibit a level of similarity of 91% with the sequence of Desulfovibrio desulfuricans ATCC 27774. These isolates were homogeneous and differed in cellular morphology, acid fastness, phenotype, electrophoretic protein profile, and habitat from Desulfovibrio species. On the basis of the results of an integrated study of the phenotype and genotype of a consistent morphological entity found in particular porcine cells and associated with a well-defined clinical condition, we concluded that these bacteria belong to a previously undescribed genus and species, for which we propose the name Lawsonia intracellularis gen. nov., sp. nov. A species-specific recombinant DNA probe was cloned previously, and this probe was used to identify the bacterium in tissue culture cells and in the ileal epithelia of pigs with proliferative enteropathy disease. Coculture of the organism with a rat enterocyte cell line allowed us to designate strain NCTC 12656 the type strain and to describe the new genus and species. The organism which we cultured is pathogenic for pigs and causes proliferative enteropathy lesions in their ilea and colons, and Koch's postulates were fulfilled for this organism.
This article has been cited by other articles:
|
|
 |
|
  S. Schmitz-Esser, I. Haferkamp, S. Knab, T. Penz, M. Ast, C. Kohl, M. Wagner, and M. Horn Lawsonia intracellularis Contains a Gene Encoding a Functional Rickettsia-Like ATP/ADP Translocase for Host Exploitation J. Bacteriol., September 1, 2008; 190(17): 5746 - 5752. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Sasi Jyothsna, Ch. Sasikala, and Ch. V. Ramana Desulfovibrio psychrotolerans sp. nov., a psychrotolerant and moderately alkaliphilic sulfate-reducing deltaproteobacterium from the Himalayas Int J Syst Evol Microbiol, April 1, 2008; 58(4): 821 - 825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Gustafsdottir, A. Nordengrahn, S. Fredriksson, P. Wallgren, E. Rivera, E. Schallmeiner, M. Merza, and U. Landegren Detection of individual microbial pathogens by proximity ligation. Clin. Chem., June 1, 2006; 52(6): 1152 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Kroll, M. A. Eichmeyer, M. L. Schaeffer, S. McOrist, D. L. Harris, and M. B. Roof Lipopolysaccharide-Based Enzyme-Linked Immunosorbent Assay for Experimental Use in Detection of Antibodies to Lawsonia intracellularis in Pigs Clin. Vaccine Immunol., June 1, 2005; 12(6): 693 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. MacIntyre, D. G. E. Smith, D. J. Shaw, J. R. Thomson, and S. M. Rhind Immunopathogenesis of Experimentally Induced Proliferative Enteropathy in Pigs Vet. Pathol., July 1, 2003; 40(4): 421 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. McCluskey, J. Hannigan, J. D. Harris, B. Wren, and D. G. E. Smith LsaA, an Antigen Involved in Cell Attachment and Invasion, Is Expressed by Lawsonia intracellularis during Infection In Vitro and In Vivo Infect. Immun., June 1, 2002; 70(6): 2899 - 2907. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. McOrist, M. Warhurst, S. McOrist, and A. R. Bird Colonic Infection by Bilophila wadsworthia in Pigs J. Clin. Microbiol., April 1, 2001; 39(4): 1577 - 1579. [Abstract] [Full Text]
|
|
|
|
|
|
L. Schoenborn, H. Abdollahi, W. Tee, M. Dyall-Smith, and P. H. Janssen A Member of the Delta Subgroup of Proteobacteria from a Pyogenic Liver Abscess Is a Typical Sulfate Reducer of the Genus Desulfovibrio J. Clin. Microbiol., February 1, 2001; 39(2): 787 - 790. [Abstract] [Full Text]
|
|
|
|
|
|
D. G. E. Smith, S. C. Mitchell, T. Nash, and S. Rhind Gamma Interferon Influences Intestinal Epithelial Hyperplasia Caused by Lawsonia intracellularis Infection in Mice Infect. Immun., December 1, 2000; 68(12): 6737 - 6743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. L. Hubbard, D. J. Harrison, C. Moyes, and S. McOrist Direct Detection of eae-Positive Bacteria in Human and Veterinary Colorectal Specimens by PCR J. Clin. Microbiol., August 1, 1998; 36(8): 2326 - 2330. [Abstract] [Full Text]
|
|
|
|
|
|
D. B. Schauer, S. N. McCathey, B. M. Daft, S. S. Jha, L. E. Tatterson, N. S. Taylor, and J. G. Fox Proliferative Enterocolitis Associated with Dual Infection with Enteropathogenic Escherichia coli and Lawsonia intracellularis in Rabbits J. Clin. Microbiol., June 1, 1998; 36(6): 1700 - 1703. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
