HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by CAYOL, J.-L. Articles by GARCIA, J.-L. Search for Related Content PubMed Articles by CAYOL, J.-L. Articles by GARCIA, J.-L. Agricola Articles by CAYOL, J.-L. Articles by GARCIA, J.-L.

Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov., Isolated from a Deep Subsurface French Oil Well, a Proposal To Reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov., and an Emended Description of Thermoanaerobacter brockii

¹ Laboratoire de Microbiologie ORSTOM, Université de Provence, 13331 Marseille Cedex 3, France
³ Sanofi Recherche, Centre de Labége, 31676 Labége Cedex, France
⁴ Unité des Entérobactéries, Institut Pasteur, 75724 Paris Cedex 15, France
² Faculty of Science and Technology, Griffith University, Nathan, Brisbane, 4111 Australia

^* Corresponding author. Mailing address: Laboratoire de Microbiologic ORSTOM, Université de Provence, Case 87, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France. Phone: 33-91-10-64-80. Fax: 33-91-10-64-81. Electronic mail address: rogerpa{at}loma.orstom.fr.

ABSTRACT

A strictly anaerobic, thermophilic, gram-positive, spore-forming eubacterium designated strain SEBR 5268^T (T = type strain) was isolated from an oil field at a depth of 2,100 m, where the temperature was 92°C. The cells of this organism were gram-positive, straight, motile rods (0.5 by 2 to 3 µm) with peritrichous flagella. The cells occurred singly or in pairs during the logarithmic growth phase, but were pleomorphic and filamentous (length, 15 µm) in old cultures. Growth occurred at temperatures of 40 to 75°C, and optimum growth occurred at temperatures between 55 and 60°C. The fermentable substrates included glucose, fructose, galactose, mannose, cellobiose, maltose, sucrose, lactose, d-xylose, d-ribose, mannitol, pyruvate, and starch. The products of fermentation of glucose were lactate, acetate, ethanol, H², and CO². The DNA base composition was 35 mol% G+C. The results of 16S rRNA sequence comparisons indicated that strain SEBR 5268^T was closely related to Thermoanaerobacter brockii and Thermoanaerobacter finnii, and these three organisms exhibited levels of ribosomal DNA sequence homology of 98 to 99%. The results of DNA-DNA hybridization studies performed with the three organisms confirmed this close affiliation, and as base pairing values of > 70% were obtained, these organisms belong to the same species. Therefore, we propose that T. finnii should be reclassified as a subspecies of T. brockii, Thermoanaerobacter brockii subsp. finnii comb. nov. This automatically creates Thermoanaerobacter brockii subsp. brockii. We also propose that strain SEBR 5268^T should be classified as a member of a new subspecies of T. brockii, Thermoanaerobacter brockii subsp. lactiethylicus. The latter differs from T. brockii subsp. brockii and T. brockii subsp. finnii by its 16S rRNA sequence, DNA sequence diversity, lower temperature optimum, G+C content, and carbohydrate utilization spectrum. Strain SEBR 5268^T has been deposited in the Deutsche Sammlung von Mikroorganismen as strain DSM 9801^T.

This article has been cited by other articles:

				R. U. Onyenwoke, V. V. Kevbrin, Anatolly. M. Lysenko, and J. Wiegel Thermoanaerobacter pseudethanolicus sp. nov., a thermophilic heterotrophic anaerobe from Yellowstone National Park Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2191 - 2193. [Abstract] [Full Text] [PDF]

				Y. Sekiguchi, H. Imachi, A. Susilorukmi, M. Muramatsu, A. Ohashi, H. Harada, S. Hanada, and Y. Kamagata Tepidanaerobacter syntrophicus gen. nov., sp. nov., an anaerobic, moderately thermophilic, syntrophic alcohol- and lactate-degrading bacterium isolated from thermophilic digested sludges. Int J Syst Evol Microbiol, July 1, 2006; 56(Pt 7): 1621 - 1629. [Abstract] [Full Text] [PDF]

				R. U. Onyenwoke, Y.-J. Lee, S. Dabrowski, B. K. Ahring, and J. Wiegel Reclassification of Thermoanaerobium acetigenum as Caldicellulosiruptor acetigenus comb. nov. and emendation of the genus description Int J Syst Evol Microbiol, June 1, 2006; 56(6): 1391 - 1395. [Abstract] [Full Text] [PDF]

				M. Bonilla Salinas, M.-L. Fardeau, P. Thomas, J.-L. Cayol, B. K. C. Patel, and B. Ollivier Mahella australiensis gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from an Australian oil well Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2169 - 2173. [Abstract] [Full Text] [PDF]

				M.-L. Fardeau, M. B. Salinas, S. L'Haridon, C. Jeanthon, F. Verhe, J.-L. Cayol, B. K. C. Patel, J.-L. Garcia, and B. Ollivier Isolation from oil reservoirs of novel thermophilic anaerobes phylogenetically related to Thermoanaerobacter subterraneus: reassignment of T. subterraneus, Thermoanaerobacter yonseiensis, Thermoanaerobacter tengcongensis and Carboxydibrachium pacificum to Caldanaerobacter subterraneus gen. nov., sp. nov., comb. nov. as four novel subspecies Int J Syst Evol Microbiol, March 1, 2004; 54(2): 467 - 474. [Abstract] [Full Text] [PDF]

				Q. Bao, Y. Tian, W. Li, Z. Xu, Z. Xuan, S. Hu, W. Dong, J. Yang, Y. Chen, Y. Xue, et al. A Complete Sequence of the T. tengcongensis Genome Genome Res., May 1, 2002; 12(5): 689 - 700. [Abstract] [Full Text] [PDF]

				Y. Takahata, M. Nishijima, T. Hoaki, and T. Maruyama Distribution and Physiological Characteristics of Hyperthermophiles in the Kubiki Oil Reservoir in Niigata, Japan Appl. Envir. Microbiol., January 1, 2000; 66(1): 73 - 79. [Abstract] [Full Text]