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Genetic Variability in the Saccharomyces Sensu Stricto Complex Revealed by Multilocus Enzyme Electrophoresis

Laboratoire d'Immunologie et de Parasitologie, UFR des Sciences Pharmaceutiques, 34060 Montpellier Cedex, France

^* Corresponding author. Mailing address: Laboratoire d'Immunologie et de Parasitologie, UFR des Sciences Pharmaceutiques, 15 av. C C. Flahault, 34060 Montpellier Cedex, France.

ABSTRACT

Fifty-two strains of the genus Saccharomyces, including 50 yeasts of the Saccharomyces sensu stricto complex (sensu Vaughan Martini 1989 [A. Vaughan Martini, Syst. Appl. Microbiol. 12:119-122, 1989]), were analyzed for nine isozyme loci with starch gel electrophoresis. The genetic analysis of isozyme data provided further evidence that the Saccharomyces sensu stricto complex is a genetically heterogeneous group. Twenty-two different electrophoretic types were identified and could be grouped into four clusters. However, no correlation between these clusters and the currently defined species Saccharomyces cerevisiae, Saccharomyces bayanus, Saccharomyces pastorianus, and Saccharomyces paradoxus was detected. Several strains could not be assigned to their putative cluster.

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