HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by ENGLE, M. Articles by WIEGEL, J. Search for Related Content PubMed Articles by ENGLE, M. Articles by WIEGEL, J. Agricola Articles by ENGLE, M. Articles by WIEGEL, J.

Isolation and Characterization of a Novel Alkalitolerant Thermophile, Anaerobranca horikoshii gen. nov., sp. nov.

¹Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, Georgia 30602
²Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

^* Corresponding author. Phone: (706) 542-2651. Fax: (706) 542-2674. Electronic mail address: jwiegel{at}uga.cc.uga.edu.

ABSTRACT

Nine moderately alkalitolerant thermophilic bacteria with similar properties were isolated from water and soil samples obtained from Yellowstone National Park. These Gram-type-positive, rod-shaped bacteria produce cells with primary branches. The cells are peritrichous and exhibit only slight tumbling motility. At 60°C the pH range for growth is 6.9 to 10.3, and the optimum pH is 8.5. At pH 8.5 the temperature range for growth is 34 to 66°C, with an optimum temperature of 57°C. The strains are mainly proteolytic. The fermentation products from yeast extract are acetate, CO₂, and H₂. Fumarate added to minimal medium containing yeast extract is stoichiometrically converted to succinate, indicating that it is used as an alternative electron acceptor. The DNA G+C content is 33 to 34 mol%. On the basis of its unique properties, such as branch formation, growth at alkaline pH values at elevated temperatures, and the relative distance of its 16S rRNA sequence from those of other known bacteria, we propose that strain JW/YL-138^T (T = type strain) and eight similar strains represent a new genus and species, Anaerobranca horikoshii. Strain JW/YL-138 is designated the type strain of the type species, A. horikoshii, which was named in honor of Koki Horikoshi, a pioneer in the field of alkaliphilic bacteria.

This article has been cited by other articles:

				V. Kevbrin, Y. Boltyanskaya, E. Garnova, and J. Wiegel Anaerobranca zavarzinii sp. nov., an anaerobic, alkalithermophilic bacterium isolated from Kamchatka thermal fields Int J Syst Evol Microbiol, June 1, 2008; 58(6): 1486 - 1491. [Abstract] [Full Text] [PDF]

				M. Ballschmiter, M. Armbrecht, K. Ivanova, G. Antranikian, and W. Liebl AmyA, an {alpha}-Amylase with {beta}-Cyclodextrin-Forming Activity, and AmyB from the Thermoalkaliphilic Organism Anaerobranca gottschalkii: Two {alpha}-Amylases Adapted to Their Different Cellular Localizations Appl. Envir. Microbiol., July 1, 2005; 71(7): 3709 - 3715. [Abstract] [Full Text] [PDF]

				V. Gorlenko, A. Tsapin, Z. Namsaraev, T. Teal, T. Tourova, D. Engler, R. Mielke, and K. Nealson Anaerobranca californiensis sp. nov., an anaerobic, alkalithermophilic, fermentative bacterium isolated from a hot spring on Mono Lake Int J Syst Evol Microbiol, May 1, 2004; 54(3): 739 - 743. [Abstract] [Full Text] [PDF]

				K. Horikoshi Alkaliphiles: Some Applications of Their Products for Biotechnology Microbiol. Mol. Biol. Rev., December 1, 1999; 63(4): 735 - 750. [Abstract] [Full Text] [PDF]