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Spiroplasma ixodetis sp. nov., a New Species from Ixodes pacificus Ticks Collected in Oregon

JOSEPH G. TULLY^1,*, DAVID L. ROSE^1,, CONRAD E. YUNKER^2,, PATRICIA CARLE³, JOSEPH M. BOVé³, DAVID L. WILLIAMSON⁴ and ROBERT F. WHITCOMB⁵

¹ Mycoplasma Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Frederick Cane Research Facility, Frederick, Maryland 21702
² Rocky Mountain Laboratory, National Institute of Allergy and Infectious Diseases, Hamilton, Montana 59840
³ Laboratoire de Biologie Cellulaire et Moléculaire, Institut Nationale de la Recherche Agronomique, and Université Bordeaux II, 33883 Villenave d'Ornon, France
⁴ Department of Anatomical Sciences, State University of New York, Stony Brook, New York 11794
⁵ Insect Pathology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

^* Corresponding author. Mailing address: Mycoplasma Section, Bldg. 550, NIAID, Frederick Cancer Res. Dev. Center, Frederick, MD 21702. Phone: (301) 846-1192. Fax: (301) 846-5165. Electronic mail address: JVT{at}CU.NIH.GOV.

ABSTRACT

Eight strains of mollicutes were isolated from pooled suspensions prepared from western black-legged ticks (Ixodes pacificus) collected in Oregon. Morphologic examination by electron and dark-field microscopic techniques showed that each strain consisted of a mixture of motile, tightly coiled helical cells, small coccoid cells with diameters ranging from 300 to 500 nm, and pleomorphic, straight or branched filamentous forms. All cellular forms were surrounded by a single cytoplasmic membrane, and there was no evidence of a cell wall. The organisms were filterable and fastidious in their growth requirements. The optimum temperature for growth was 30°C, but multiplication occurred at temperatures ranging from 23 to 32°C. The strains catabolized glucose but did not hydrolyze arginine or urea. The genome size of strain Y32^T (T = type strain) was 2,220 kbp, and the DNA base composition (guanine-plus-cytosine content) of this organism was 25 ± 1 mol%. The eight isolates were serologically related to each other but were not related to 37 other type or representative strains belonging to the genus Spiroplasma. Strain Y32 (= ATCC 33835) is the type strain of Spiroplasma ixodetis sp. nov.

Present address: 8602 Cinnamon Creek, Apt. 209, San Antonio, TX 78240.

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