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Heterogeneity of Genome Sizes within the Genus Spiroplasma

¹ Laboratoire de Biologie Cellulaire et Moléculaire, Institut National de la Recherche Agronomique, and Universití de Bordeaux II, 33883 Villenave d' Ornon Cedex, France
² Mycoplasma Section, Frederick Cancer Research and Development Center, National Institute of Allergy and Infectious Diseases, Frederick, Maryland 21702

^* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux II, BP81, 33883 Villenave d'Ornon Cedex, France. Phone: 33.56.84.31.50. Fax: 33.56.84.31.59. Electronic mail address: laigret{at}bordeaux.inra.fr.

ABSTRACT

Organisms belonging to the genus Spiroplasma are currently classified into 23 groups, 17 of which have been assigned species epithets. We determined the genome sizes of representatives of 20 groups by using pulsed-field gel electrophoresis. Each genome size was deduced from the mobility of linear nonrestricted DNA, as well as from the sum of the sizes of restriction fragments obtained after digestion with Not I, a restriction endonuclease with a limited number of restriction sites in spiroplasma DNA. The values which we obtained indicated that the genome sizes of members of the genus Spiroplasma range from 940 to 2,220 kbp.

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