HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by CHEN, W. Articles by LI, Y. Search for Related Content PubMed PubMed Citation Articles by CHEN, W. Articles by LI, Y. Agricola Articles by CHEN, W. Articles by LI, Y.

Characteristics of Rhizobium tianshanense sp. nov., a Moderately and Slowly Growing Root Nodule Bacterium Isolated from an Arid Saline Environment in Xinjiang, People's Republic of China

Department of Microbiology, Beijing Agricultural University, Beijing 100094, People's Republic of China

^* Corresponding author. Telex: 222487 BAU CN. Fax: 86-1-2582332.

ABSTRACT

We performed a numerical analysis of 148 phenotypic characteristics of 20 strains of root nodule bacteria isolated from an arid saline desert soil in the Xinjiang region of northwestern People's Republic of China and compared these organisms with 28 Rhizobium and Bradyrhizobium strains obtained from different regions of the People's Republic of China and from other countries, including nine type strains of different species. All of the strains examined clustered into two groups at a similarity level of more than 63%. Group I included all of the previously described Rhizobium species and was divided into eight subgroups, which corresponded to previously described Rhizobium species, at a similarity level of more than 82%. Group II was divided into the following three subgroups at a similarity level of more than 80% Bradyrhizobium japonicum, a cluster containing 17 moderately and slowly growing strains isolated in the Xinjiang region, and a small subgroup containing three fast-growing strains. The generation times of the moderately and slowly growing strains were 5 to 15 h, and these organisms produced acid in medium containing mannitol. The DNA G+C contents of the members of this group ranged from 59 to 63 mol%. DNA-DNA hybridization experiments revealed that the levels of DNA homology among all of the moderately and slowly growing strains obtained from Xinjiang were more than 70% and that the levels of DNA homology between representative strains of this group and the type strains of all previously described species of root- and stem-nodulating bacteria were low. All of our experimental data indicated that the moderately and slowly growing rhizobia isolated from Xinjiang are members of a new species. Partial 16S rRNA gene sequencing of the type strain. A-1BS (=CCBAU3306), and a comparison of the resulting sequence with the sequences of previously described species revealed that strain A-1BS is closely related to Rhizobium loti, Rhizobium huakuii, and Rhizobium galegae, but not to B. japonicum and Azorhizobium caulinodans. On the basis of our results, we propose that the strains which we studied are members of a new species, Rhizobium tianshanense. The type strain, A-1BS (= CCBAU3306), has been deposited in the Culture Collection of Beijing Agricultural University, Beijing, People's Republic of China.

This article has been cited by other articles:

				C. Vidal, C. Chantreuil, O. Berge, L. Maure, J. Escarre, G. Bena, B. Brunel, and J.-C. Cleyet-Marel Mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of Anthyllis vulneraria growing on metallicolous soil in Languedoc, France Int J Syst Evol Microbiol, April 1, 2009; 59(4): 850 - 855. [Abstract] [Full Text] [PDF]

				T. X. Han, L. L. Han, L. J. Wu, W. F. Chen, X. H. Sui, J. G. Gu, E. T. Wang, and W. X. Chen Mesorhizobium gobiense sp. nov. and Mesorhizobium tarimense sp. nov., isolated from wild legumes growing in desert soils of Xinjiang, China Int J Syst Evol Microbiol, November 1, 2008; 58(11): 2610 - 2618. [Abstract] [Full Text] [PDF]

				S. H. Guan, W. F. Chen, E. T. Wang, Y. L. Lu, X. R. Yan, X. X. Zhang, and W. X. Chen Mesorhizobium caraganae sp. nov., a novel rhizobial species nodulated with Caragana spp. in China Int J Syst Evol Microbiol, November 1, 2008; 58(11): 2646 - 2653. [Abstract] [Full Text] [PDF]

				C. Appunu, A. N'Zoue, and G. Laguerre Genetic Diversity of Native Bradyrhizobia Isolated from Soybeans (Glycine max L.) in Different Agricultural-Ecological-Climatic Regions of India Appl. Envir. Microbiol., October 1, 2008; 74(19): 5991 - 5996. [Abstract] [Full Text] [PDF]

				T. X. Han, E. T. Wang, L. J. Wu, W. F. Chen, J. G. Gu, C. T. Gu, C. F. Tian, and W. X. Chen Rhizobium multihospitium sp. nov., isolated from multiple legume species native of Xinjiang, China Int J Syst Evol Microbiol, July 1, 2008; 58(7): 1693 - 1699. [Abstract] [Full Text] [PDF]

				F. Q. Wang, E. T. Wang, J. Liu, Q. Chen, X. H. Sui, W. F. Chen, and W. X. Chen Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China Int J Syst Evol Microbiol, June 1, 2007; 57(6): 1192 - 1199. [Abstract] [Full Text] [PDF]

				H. Zheng, Z. Zhong, X. Lai, W.-X. Chen, S. Li, and J. Zhu A LuxR/LuxI-Type Quorum-Sensing System in a Plant Bacterium, Mesorhizobium tianshanense, Controls Symbiotic Nodulation. J. Bacteriol., March 1, 2006; 188(5): 1943 - 1949. [Abstract] [Full Text] [PDF]

				J.-L. Gao, S. L. Turner, F. L. Kan, E. T. Wang, Z. Y. Tan, Y. H. Qiu, J. Gu, Z. Terefework, J. P. W. Young, K. Lindstrom, et al. Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China Int J Syst Evol Microbiol, November 1, 2004; 54(6): 2003 - 2012. [Abstract] [Full Text] [PDF]

				N. Labbe, S. Parent, and R. Villemur Nitratireductor aquibiodomus gen. nov., sp. nov., a novel {alpha}-proteobacterium from the marine denitrification system of the Montreal Biodome (Canada) Int J Syst Evol Microbiol, January 1, 2004; 54(1): 269 - 273. [Abstract] [Full Text] [PDF]

				G. H. Wei, Z. Y. Tan, M. E Zhu, E. T. Wang, S. Z. Han, and W. X. Chen Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. nov. Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1575 - 1583. [Abstract] [Full Text] [PDF]

				G. Laguerre, S. M. Nour, V. Macheret, J. Sanjuan, P. Drouin, and N. Amarger Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts Microbiology, April 1, 2001; 147(4): 981 - 993. [Abstract] [Full Text]

				E. Velázquez, J. M. Cruz-Sánchez, P. F. Mateos, and E. Martínez-Molina Analysis of Stable Low-Molecular-Weight RNA Profiles of Members of the Family Rhizobiaceae Appl. Envir. Microbiol., April 1, 1998; 64(4): 1555 - 1559. [Abstract] [Full Text]