HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by ARTIUSHIN, S. Articles by MINION, F. C. Search for Related Content PubMed Articles by ARTIUSHIN, S. Articles by MINION, F. C. Agricola Articles by ARTIUSHIN, S. Articles by MINION, F. C.

Phylogenetic Analysis of Mycoplasma Strain ISM1499 and Its Assignment to the Acholeplasma oculi Strain Cluster

¹ Veterinary Medical Research Institute, Iowa State University, Ames, Iowa 50011
² Department of Biology and Microbiology, South Dakota State University, Brookings, South Dakota 57077–0595

^* Corresponding author. Mailing address: Veterinary Medical Research Institute, Iowa State University, 1802 Elwood Drive, Ames, IA 50011. Phone: (515) 294-6347. Fax: (515) 294-1401. Electronic mail address: fcminion{at}iastate.edu.

ABSTRACT

A mycoplasma strain designated ISM1499 was used to develop a mycoplasma genetic system (G. G. Mahairas and F. C. Minion, J. Bacteriol. 171:1775–1780, 1989; G. G. Mahairas, C. Jian, and F. C. Minion, Gene 93:61–65, 1990), but phenotypic inconsistencies led to the conclusion that this organism had been classified incorrectly as a member of the species Mycoplasma pulmonis. Studies were initiated to determine the proper taxonomic position of ISM1499, and on the basis of the results of our genetic analysis, this strain was assigned to the Acholeplasma oculi strain cluster. The base composition of strain ISM1499 was identical to the base composition of A oculi 19L, but not to the base composition of Acholeplasma laidlawii PG8 (28.3 and 30.7 mol% G+C, respectively). The taxonomic position of ISM1499 was examined by performing a parsimony analysis with 16S ribosomal DNA sequence data, and the results were compared with previous phylogenetic reconstructions. Our results indicated that ISM1499 is more closely related phylogenetically to A. oculi 19L than to A. laidlawii PG8 and JA1. Heterogeneity in the 16S ribosomal DNA sequences of A. oculi 19L and ISM1499 and in the 16S ribosomal DNA sequences of A. laidlawii PG8 and JA1 may indicate that unusual dissimilarities occur in the 16S ribosomal DNA sequences of members of the genus Acholeplasma.

This article has been cited by other articles:

				F. C. Minion, E. J. Lefkowitz, M. L. Madsen, B. J. Cleary, S. M. Swartzell, and G. G. Mahairas The Genome Sequence of Mycoplasma hyopneumoniae Strain 232, the Agent of Swine Mycoplasmosis J. Bacteriol., November 1, 2004; 186(21): 7123 - 7133. [Abstract] [Full Text] [PDF]

				C. M. M. Cordova, C. Lartigue, P. Sirand-Pugnet, J. Renaudin, R. A. F. Cunha, and A. Blanchard Identification of the Origin of Replication of the Mycoplasma pulmonis Chromosome and Its Use in oriC Replicative Plasmids J. Bacteriol., October 1, 2002; 184(19): 5426 - 5435. [Abstract] [Full Text] [PDF]

				T. Yoshida, S.-I. Maeda, T. Deguchi, and H. Ishiko Phylogeny-Based Rapid Identification of Mycoplasmas and Ureaplasmas from Urethritis Patients J. Clin. Microbiol., January 1, 2002; 40(1): 105 - 110. [Abstract] [Full Text] [PDF]

				K. J. Jarvill-Taylor, C. VanDyk, and F. C. Minion Cloning of mnuA, a Membrane Nuclease Gene of Mycoplasma pulmonis, and Analysis of Its Expression in Escherichia coli J. Bacteriol., March 15, 1999; 181(6): 1853 - 1860. [Abstract] [Full Text]