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Rapid Characterization of Four Species of the Saccharomyces Sensu Stricto Complex According to Mitochondrial DNA Patterns

JOSÉ MANUEL GUILLAMON^1,, ELADIO BARRIO², TOMÁS HUERTA¹ and AMPARO QUEROL^1,*

¹Departamento de Microbiología, Facultad de Ciencias Biológicas, Universitat de València, 46100-Burjasot, Valencia, Spain
²Departamento de Genética Facultad de Ciencias Biológicas, Universitat de València, 46100-Burjasot, Valencia, Spain

^* Corresponding author. Mailing address: Departamento de Biotecnologia, Institute de Agroquímica y Tecnología de Alimentos, C.S.I.C., c/Jaime Roig 11, 46010 Valencia, Spain. Phone: 34-6-369-0800. Fax: 34-6-393-0001. Electronic mail address: AmparoQuerolebamo{at}evalsb.geneti.uv.es.

ABSTRACT

Several strains of the four sibling species of the genus Saccharomyces (S. bayanus, S. cerevisiae, S. paradoxus, and S. pastorianus) were characterized by using a rapid and simple method of restriction analysis of mitochondrial DNA. Patterns obtained with four-cutter endonucleases (such as AluI, DdeI, HinfI, and RsaI) made it possible to differentiate each species. S. cerevisiae and S. paradoxus presented a greater number of large fragments than S. pastorianus and S. bayanus with all the assay enzymes. With AluI and DdeI, species-specific bands clearly permitted differentiation between S. pastorianus and S. bayanus. To test the resolution of this method, wild Saccharomyces strains were analyzed. The correct assignment of these strains to a known taxon by this rapid method was confirmed by means of electrophoretic karyotyping.

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