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Pulsed-Field Gel Electrophoresis of Genomic Digests of Thermus Strains and Its Implications for Taxonomic and Evolutionary Studies

¹ Experimental Biology Research Group, School of Biological Sciences, University of Auckland, Auckland, New Zealand
³ Centre for Gene Technology, University of Auckland, Auckland, New Zealand
² Center for Marine Biotechnology, Baltimore, Maryland 21202

^* Corresponding author. Mailing address: Centre for Gene Technology, University of Auckland, Private Bag 92019, Auckland, New Zealand. Phone: 64-9-3737599, ext. 7712. Fax: 64-9-3737414.

ABSTRACT

Pulsed-field electrophoresis (PFGE) of the Ssp I genomic digests of 14 Thermus isolates showed that each one had a unique restriction enzyme digestion pattern. A group of New Zealand strains showed some shared bands, but each isolate gave essentially a unique fingerprint. In addition, evolutionary distances between Thermus strains estimated by using PFGE restriction fragment length polymorphisms (PFGE-RFLPs) correlate well with those based on small-subunit rRNA sequence data. As a consequence, the phylogenetic trees constructed on the basis of PFGE-RFLPs and those constructed by using small-subunit rRNA sequences generally agree. On the basis of the evolutionary distances estimated by using PFGE-RFLPs, the estimated average genomic rate of divergence for Thermus spp. is approximately 0.27% per million years.

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