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Classification of Leptospires of the Pyrogenes Serogroup Isolated from Cattle in Zimbabwe by Cross-Agglutinin Absorption and Restriction Fragment Length Polymorphism Analysis

¹ Department of Biological Sciences, University of Zimbabwe, Mount Pleasant, Harare, Zimbabwe
² Leptospirosis and Mycobacteriosis Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 50010
³ and N. H. Swellengrebel Laboratory of Tropical Hygiene, The Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands

^* Corresponding author. Mailing address: Department of Biological Sciences, University of Zimbabwe, MP167, Mount Pleasant, Harare, Zimbabwe. Phone: 263-4-303211. Fax: 263-4-333407.

ABSTRACT

Five strains of the genus Leptospira belonging to serogroup Pyrogenes were isolated from cattle slaughtered in Zimbabwe and subjected to cross-agglutinin absorption and restriction fragment length polymorphism analysis. One strain, SBF 2, represents a new genetic strain of serovar kwale, while another strain, SBF 49, is a new genetic strain closely related to serovar nigeria. Three strains belong to a new serovar for which the name mombe with reference strain SBF 20 is proposed. Restriction fragment length polymorphism analysis indicated that each of these three strains represents a different restriction polymorphism pattern group.

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