HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Erratum (v45,p201) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by CAYOL, J.-L. Articles by GARCIA, J.-L. Search for Related Content PubMed Articles by CAYOL, J.-L. Articles by GARCIA, J.-L. Agricola Articles by CAYOL, J.-L. Articles by GARCIA, J.-L.

Isolation and Characterization of Halothermothrix orenii gen. nov., sp. nov., a Halophilic, Thermophilic, Fermentative, Strictly Anaerobic Bacterium

¹ Laboratoire de Microbiologie, ORSTOM, Université de Provence, 13331 Marseille cedex 3, France
³ Laboratoire de Microbiologie, Université Blaise Pascal, 63177 Aubière cedex, France
⁴ Laboratoire de Chimie et Corrosion Marine, IFREMER, 29273 Brest cedex, France
² Faculty of Science and Technology, Griffith University, Brisbane, Queensland 4111, Australia

^* Corresponding author. Mailing address: Laboratoire de microbiologie, ORSTOM, Case 87, Université de Provence, 3 Pl. Victor Hugo, 13331 Marseille Cedex 03, France.

ABSTRACT

The occurrence of thermophilic, halophilic anaerobic bacteria in the sediment of a Tunisian salted lake was tested in samples collected at 20-cm intervals down to a depth of 1.20 m. A long rod, present only in the 40-to 60-cm layer, was isolated at 60°C in a medium containing 100 g of NaCl per liter and designated strain H168. This strain produced acetate, ethanol, H₂, and CO₂ from glucose metabolism. Fructose, xylose, ribose, cellobiose, and starch were also oxidized. The optimum temperature for growth was 60°C. No growth was obtained at 42 or 70°C. Strain H168 grew optimally in NaCl concentrations ranging from 50 to 100 g per liter, with the upper and lower limits of growth around 200 and 40 g per liter, respectively. The G+C ratio of the DNA was 39.6 mol %. Although halophilic, moderately thermophilic bacteria have been characterized among anaerobes, particularly within methanogens, strain H168 is the first true thermophilic (growing above 60°C) halophilic anaerobic bacterium described so far. The phylogeny, physiology, morphology, lipid content, and high G+C content of strain H168 are sufficiently different from those of genera belonging to the family Haloanaerobiaceae to justify the definition of a new genus.

This article has been cited by other articles:

				T. K. Chua, J. M. Bujnicki, T.-C. Tan, F. Huynh, B. K. Patel, and J. Sivaraman The Structure of Sucrose Phosphate Synthase from Halothermothrix orenii Reveals Its Mechanism of Action and Binding Mode PLANT CELL, April 1, 2008; 20(4): 1059 - 1072. [Abstract] [Full Text] [PDF]

				B. N. Mijts and B. K. C. Patel Cloning, sequencing and expression of an {alpha}-amylase gene, amyA, from the thermophilic halophile Halothermothrix orenii and purification and biochemical characterization of the recombinant enzyme Microbiology, August 1, 2002; 148(8): 2343 - 2349. [Abstract] [Full Text] [PDF]

				W. Eder, L. L. Jahnke, M. Schmidt, and R. Huber Microbial Diversity of the Brine-Seawater Interface of the Kebrit Deep, Red Sea, Studied via 16S rRNA Gene Sequences and Cultivation Methods Appl. Envir. Microbiol., July 1, 2001; 67(7): 3077 - 3085. [Abstract] [Full Text] [PDF]