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1 Department of Population Health and Reproduction, University of California School of Veterinary Medicine, Davis, California 95616
2 Mycoplasma Section, Frederick Cancer Research and Development Center, National Institute of Allergy and Infectious Diseases, Frederick, Maryland 21702
3 Respiratory and Systemic Infections Laboratory and National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, England
4 Animal Health Research Laboratory, CSIRO Division of Animal Health, Parkville, Victoria 3052, Australia
* Corresponding author. Phone: (916) 752-7517. Fax: (916) 752-4278.
ABSTRACT
Three mycoplasma strains, designated GIHT (T = type strain), UIAT, and VISTT, were isolated from the external ear canals of goats and were shown to be serologically distinct from each other and from previously described Acholeplasma, Entomoplasma, Mesoplasma, and Mycoplasma species. Using light and transmission electron microscopy, we showed that the cells of these organisms were small, pleomorphic, coccoid, nonmotile, and nonhelical and that each cell was surrounded by a single cytoplasmic membrane. There was no evidence of a cell wall, and the organisms grew freely in media containing penicillin at concentrations of 1,000 U/ml or more and thallous acetate (final concentration, 1:4,000) and produced the "fried-egg" morphology typical of most mollicutes. Growth occurred both aerobically and anaerobically (as determined by the GasPak method). The ability to catabolize glucose and mannose and the ability to hydrolyze arginine varied among the three strains. All three strains required sterol for growth, and none of the strains hydrolyzed urea. The guanine-plus-cytosine contents of the DNAs of strains UIAT, VIST, and GIHT were determined to be 26.9, 27.0, and 26.6 mol%, respectively. Our data indicate that the three strains represent new Mycoplasma species, for which we propose the names Mycoplasma auris, Mycoplasma cottewii, and Mycoplasma yeatsii. The type strain of M. auris is UIA (= ATCC 51348 = NCTC 11731), the type strain of M. cottewii is VIS (= ATCC 51347 = NCTC 11732), and the type strain of M. yeatsii is GIH (= ATCC 51346 = NCTC 11730).
Present address: 13 Oakland Court, Burleigh Waters, Queensland 4220, Australia.
Present address: 8602 Cinnamon Creek, San Antonio, TX 78240.
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