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Int J Syst Bacteriol 44 (1994), 362-364; DOI 10.1099/00207713-44-2-362
© 1994 Society for General Microbiology
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A New Serovar in the Grippotyphosa Serogroup Comprising Leptospiral Isolates from Different Regions

J. L. HERRMANN1, P. BAKOSS2, H. KORVER3, A. A. BULU4, E. BELLENGER1, W. J. TERPSTRA3, I. SAINT GIRONS1 and G. BARANTON1,*

1 Unité de Bactériologie Moléculaire et Médicate, Institut Pasteur, 75724 Paris Cédex 15, France
2 Department of Epidemiology, Medical Faculty of Komensky-University, 81108 Bratislava, Slovakia
3 N. H. Swellengrebel Laboratory of Tropical Hygiene, The Royal Tropical Institute, Amsterdam, The Netherlands
4 Veteriner Kontrol ve Arastirma Enstitusu, 06020 Etlik-Ankara, Turkey

* Corresponding author. Mailing address: Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 83 37. Fax: 33 1 40 61 30 01. Electronic mail address: gbaran{at}pasteur.fr.

ABSTRACT

Pulsed-field gel electrophoresis (PFGE) studies performed with leptospiral isolates led us to suspect the existence of a new serovar in the Grippotyphosa serogroup. The results obtained with reference serological techniques used in leptospiral identification, including cross-agglutination absorption and monoclonal antibody techniques, confirmed the existence of a new serovar exemplified by strain Dadas I. Four other isolates from different regions of the world were submitted for identification by PFGE and produced Not I restriction patterns similar to that of strain Dadas I. Our data demonstrate the power of PFGE for identifying leptospiral isolates. The name dadas is proposed for the new serovar.




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