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Porphyromonas canoris sp. nov., an Asaccharolytic, Black-Pigmented Species from the Gingival Sulcus of Dogs

¹ Department of Veterinary Pathology, University of Sydney, Sydney, New South Wales 2006, Australia
² Anaerobe Reference Laboratory, National Public Health Institute, 00300 Helsinki, Finland
³ College of Veterinary Medicine, 00530 Helsinki, Finland

^* Corresponding author. Mailing address: Department of Veterinary Pathology, Sydney University, New South Wales 2006, Australia. Phone: 61-2-692-2454. Fax: 61-2-552-6526. Electronic mail address: suvp00{at}angis.su.oz.au.

ABSTRACT

A new species, Porphyromonas canoris, is proposed for black-pigmented asaccharolytic strains isolated from subgingival plaque samples from dogs with naturally occurring periodontal disease. This bacterium is an obligately anaerobic, nonmotile, non-spore-forming, gram-negative, rod-shaped organism. On laked rabbit blood or sheep blood agar plates, colonies are light brown to greenish brown after 2 to 4 days of incubation and dark brown after 14 days of incubation. Colonies on egg yolk agar and on nonhemolyzed sheep blood agar are orange. The cells do not grow in the presence of 20% bile and have a guanine-plus-cytosine content of 49 to 51 mol%. The type strain is VPB 4878 (= NCTC 12835). The average levels of DNA-DNA hybridization between P. canoris strains and other members of the genus Porphyromonas are as follows: Porphyromonas gingivalis ATCC 33277^T (T = type strain), 6.5%; Porphyromonas gingivalis cat strain VPB 3492, 5%; Porphyromonas endodontalis ATCC 35406^T, 1%; Porphyromonas salivosa NCTC 11362^T, 5%; and Porphyromonas circumdentaria NCTC 12469^T, 6%. The level of hybridization between P. canoris NCTC 12835^T DNA and Porphyromonas asaccharolytica ATCC 25260^T DNA is 3%. P. canoris cells produce major amounts of acetic, propionic, isovaleric, and succinic acids and minor amounts of isobutyric and butyric acids as end products of metabolism in cooked meat medium. The major cellular fatty acid is 13-methyltetradecanoic acid (iso-C_15:0). Glutamate and malate dehydrogenases are present, as are glucose-6-phosphate dehydrogenase activity (65.7 nmol mg of protein^-1 min^-1) and 6-phosphogluconate dehydrogenase activity (63.0 nmol mg of protein^-1 min^-1). P. canoris cells do not agglutinate sheep erythrocytes but exhibit brick red fluorescence at 265 nm and produce catalase.

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