| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Molecular Microbial Ecology, Institute of Virology and Environmental Microbiology, Oxford OX1 3SR, United Kingdom
2 Department of Microbiology, New York University Medical Center, New York, New York 10016
* Corresponding author. Mailing address: Molecular Microbial Ecology, Institute of Virology & Environmental Microbiology, Mansfield Road, Oxford OX1 3SR, United Kingdom. Phone: (0)865 512361. Fax: (0)865 59962. Electronic mail address: Paul.Raney{at}St-Cross.Oxford.ac.uk.
ABSTRACT
A genome and fatty acid analysis of 16 Pseudomonas stutzeri reference strains having DNA compositions ranging from 62.2 to 65.5 mol% G+C was performed by pulsed-field gel electrophoresis of Xba I and Spe I macrorestriction fragments and gas chromatography of total cellular fatty acids. Macrorestriction fragment patterns were evaluated by using previously described algorithms (D. Grothues and B. Tümmler, Mol. Microbiol. 5:2763-2776, 1991), and the results allowed us to subdivide the species into two groups which correlated with G+C content. Two examples of recent strain divergence were observed among clinical isolates, but in general a marked degree of heterogeneity was observed in the macrorestriction fragment patterns, and even phenotypically similar strains produced divergent patterns. While the differences were not sufficiently great to exclude any strain from P. stutzeri, they suggest that recombination and niche-specific selection may be significant factors responsible for generating and maintaining the heterogeneity inherent in the species. Genome sizes were estimated from the sums of Spe I restriction fragment sizes and ranged from 3.4 to 4.3 Mbp; the genome sizes of the low-G+C-content strains (G+C contents, approximately 62 mol%) were confined to a narrow range between 3.9 and 4.1 Mbp. An examination of the distributions of macrorestriction fragments resulting from digestion with Xba I and Spe I showed that both distributions differed significantly from the expected (random) distribution, suggesting that there is a supragenic level of chromosomal organization. An analysis of fatty acid methyl ester data by using Microbial Identification System software revealed a similar correlation between phenotype and G+C content, indicating that division of the species is possible by the method used in this study. For comparative purposes, a numerical analysis of previously reported substrate utilization data (N. J. Palleroni, M. Doudoroff, R. Y. Stanier, R. E. Solánes, and M. Mandel, J. Gen. Microbiol. 60:215-231, 1970) was performed. The results of this analysis revealed that there was a relationship among strains which showed no correlation with the results obtained from either the macrorestriction fragment analysis or the fatty acid methyl ester analysis.
This article has been cited by other articles:
|
|
 |
|
  Q. Lai and Z. Shao Pseudomonas xiamenensis sp. nov., a denitrifying bacterium isolated from activated sludge Int J Syst Evol Microbiol, August 1, 2008; 58(8): 1911 - 1915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lalucat, A. Bennasar, R. Bosch, E. Garcia-Valdes, and N. J. Palleroni Biology of Pseudomonas stutzeri Microbiol. Mol. Biol. Rev., June 1, 2006; 70(2): 510 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Romanenko, M. Uchino, E. Falsen, G. M. Frolova, N. V. Zhukova, and V. V. Mikhailov Pseudomonas pachastrellae sp. nov., isolated from a marine sponge Int J Syst Evol Microbiol, March 1, 2005; 55(2): 919 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. L. Barragan, M. Carmona, M. T. Zamarro, B. Thiele, M. Boll, G. Fuchs, J. L. Garcia, and E. Diaz The bzd Gene Cluster, Coding for Anaerobic Benzoate Catabolism, in Azoarcus sp. Strain CIB J. Bacteriol., September 1, 2004; 186(17): 5762 - 5774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sikorski, N. Teschner, and W. Wackernagel Highly Different Levels of Natural Transformation Are Associated with Genomic Subgroups within a Local Population of Pseudomonas stutzeri from Soil Appl. Envir. Microbiol., February 1, 2002; 68(2): 865 - 873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Rius, M. C. Fusté, C. Guasp, J. Lalucat, and J. G. Lorén Clonal Population Structure of Pseudomonas stutzeri, a Species with Exceptional Genetic Diversity J. Bacteriol., January 15, 2001; 183(2): 736 - 744. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. G. Lorenz and J. Sikorski The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri Microbiology, December 1, 2000; 146(12): 3081 - 3090. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
