HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by LI, Y. Articles by WIEGEL, J. Search for Related Content PubMed Articles by LI, Y. Articles by WIEGEL, J. Agricola Articles by LI, Y. Articles by WIEGEL, J.

Clostridium thermoalcaliphilum sp. nov., an Anaerobic and Thermotolerant Facultative Alkaliphile

¹ Department of Microbiology and Center for Biological Resource Recovery, University of Georgia, Athens, Georgia 30602
² German Collection of Microorganisms and Cell Cultures, 3300 Braunschweig, Germany
³ Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801

^* Corresponding author. Fax: (706) 542-2605. Electronic mail address: jwiegel{at}uga.cc.uga.edu.

ABSTRACT

An anaerobic and thermophilic alkaliphile, strain JW/YL23-2^T (T = type strain), was isolated from sewage sludge obtained from a sewage plant in Atlanta, Ga. At pH 10.1 and 50°C, the doubling time of this strain was 19 min. Strain JW/YL23-2^T, a motile rod-shaped bacterium with 2 to 12 peritrichous flagella, exhibited a negative Gram stain reaction but was gram-type positive as judged by the polymyxin B test. No heat-stable (85°C, 15 min) endospores were detected. At 50°C, growth occurred at pH values ranging from 7.0 to 11.0; the optimum pH was 9.6 to 10.1. The temperature range for growth ranged from 27 to 57.5°C; the optimum temperature was 48 to 51°C (pH 10.1). Dissimilatory sulfate reduction was not detected. The organism utilized glucose, fructose, sucrose, maltose, cellobiose, and Casamino Acids. The DNA G+C content was 32 mol% (as determined by chemical analysis). A 16S rRNA sequence analysis revealed a 2% inferred evolutionary distance to Clostridium paradoxum. However, the cell wall type of strain JW/YL23-2^T was A4β (L-Orn-D-Asp), while that of C. paradoxum was A1(m-diaminopimelic acid direct). On the basis of the alkaline pH values and high temperatures for optimal growth, the inability to form spores, and other characteristics different from C. paradoxum characteristics, strain JW/YL-23-2 was placed in a new species, Clostridium thermoalcaliphilum; JW/YL23-2 (= DSM 7309) is the type strain of this new species.

This article has been cited by other articles:

				S. Chen, L. Song, and X. Dong Sporacetigenium mesophilum gen. nov., sp. nov., isolated from an anaerobic digester treating municipal solid waste and sewage. Int J Syst Evol Microbiol, April 1, 2006; 56(Pt 4): 721 - 725. [Abstract] [Full Text] [PDF]

				L. Urios, V. Cueff, P. Pignet, and G. Barbier Tepidibacter formicigenes sp. nov., a novel spore-forming bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent Int J Syst Evol Microbiol, March 1, 2004; 54(2): 439 - 443. [Abstract] [Full Text] [PDF]

				A. I. Slobodkin, T. P. Tourova, N. A. Kostrikina, N. A. Chernyh, E. A. Bonch-Osmolovskaya, C. Jeanthon, and B. E. Jones Tepidibacter thalassicus gen. nov., sp. nov., a novel moderately thermophilic, anaerobic, fermentative bacterium from a deep-sea hydrothermal vent Int J Syst Evol Microbiol, July 1, 2003; 53(4): 1131 - 1134. [Abstract] [Full Text] [PDF]

				K. Horikoshi Alkaliphiles: Some Applications of Their Products for Biotechnology Microbiol. Mol. Biol. Rev., December 1, 1999; 63(4): 735 - 750. [Abstract] [Full Text] [PDF]

				C. J. Peddie, G. M. Cook, and H. W. Morgan Sodium-Dependent Glutamate Uptake by an Alkaliphilic, Thermophilic Bacillus Strain, TA2.A1 J. Bacteriol., May 15, 1999; 181(10): 3172 - 3177. [Abstract] [Full Text]