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Phylogenetic Relationship of Chlamydia pneumoniae to Chlamydia psittaci and Chlamydia trachomatis as Determined by Analysis of 16S Ribosomal DNA Sequences

¹Division of Infectious Diseases, Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205-2196
⁵Division of Pediatric Infectious Diseases, Department of Pediatrics Department of Medicine, The Johns Hopkins University, Baltimore, Maryland 21205-2196
²Department of Microbiology and Immunology, Stanford University, Stanford, California 94305
³Department of Medicine, School of Veterinary Medicine, University of California-Davis, Davis, California 95616
⁴National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892

^* Corresponding author.

ABSTRACT

The 16S ribosomal DNA sequence of Chlamydia pneumoniae was determined and compared with the corresponding gene sequences of Chlamydia psittaci and Chlamydia trachomatis. C. pneumoniae has been reported to exhibit little chromosomal DNA homology with the other chlamydial species, and its phylogenetic relationships within the genus Chlamydia have not been described. A polymerase chain reaction was employed to determine the 16S rRNA gene sequence of C. pneumoniae. Ten primers from the C. psittaci sequences were used to amplify a C. pneumoniae template in overlapping segments of the gene. Sequence data for 1,554 bases indicated that the levels of homology of C. pneumoniae with C. psittaci and C. trachomatis were 96.19 and 94.07%, respectively. These data support the results of previous biochemical and developmental studies indicating that C. pneumoniae is more closely related to C. psittaci than to C. trachomatis.

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