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1Laboratoire de Biologie Cellulaire et MolÉculaire, Domaine de la Grande Ferrade, Institut National de la Recherche Agronomique et UniversitÉ de Bordeaux II, F-33883 Villenave d'Ornon, France
2Mycoplasma Reference Laboratory, National Collection of Type Cultures, Public Health Laboratory Service, London, England NW9 5HT
3Mycoplasma Section National Institute of Allergy and Infectious Diseases, Frederick, Maryland 21702
4Division of Animal Health, Commonwealth Scientific and Industrial Research Organization, Parkville, Victoria, Australia
* Corresponding author.
ABSTRACT
DNA-DNA hybridization experiments were carried out in order to clarify the taxonomic relationships between the F38 group of caprine mycoplasmas, the established etiologic agents of classical contagious caprine pleuropneumonia, and Mycoplasma capricolum, an organism associated with septicemia, arthritis, and mastitis in goats and sheep. The taxonomic status of the F38 group has been uncertain, principally because of the serological, genomic, and other properties which it shares with M. capricolum. Tritium-labeled DNAs from the M. capricolum type strain (California kid) and from prototype strain F38 were hybridized with unlabeled DNAs from these two strains and from four other isolates belonging to each group. The results showed consistent DNA relatedness values of about 70% between the F38 and M. capricolum groups, compared with levels of relatedness of about 90 and 85%, respectively, for the strains within each group. In addition, the results of comparisons of these 10 strains in which growth inhibition and immunofluorescence tests were used confirmed the previously reported serological relationships between the two groups and reinforced other observations concerning their shared genomic and cell membrane characteristics, indicating that there is a close taxonomic relationship. However, as the 70% DNA relatedness values between the M. capricolum and F38 groups also indicate a degree of genomic difference inconsistent with a relationship at the species level, we conclude that our findings support previous proposals for classification of the F38 group as a subspecies of M. capricolum. In view of the prospective diagnostic problems, particularly those arising from the serological similarities of two putative subspecies, we believe that further studies should be performed to define additional phenotypic and genotypic properties that would allow more rapid and specific differentiation of the F38 group mycoplasmas.
Present address: Number 13, Oakland Court, Burleigh Waters, Queensland 4200, Australia. This article has been cited by other articles:
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  D. R. Brown, R. F. Whitcomb, and J. M. Bradbury Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes) Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2703 - 2719. [Abstract] [Full Text] [PDF]
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 S. P. Djordjevic, E. M. Vilei, and J. Frey Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (gtsABC) Microbiology, January 1, 2003; 149(1): 195 - 204. [Abstract] [Full Text] [PDF]
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 B. Pettersson, G. Bölske, F. Thiaucourt, M. Uhlén, and K.-E. Johansson Molecular Evolution of Mycoplasma capricolum subsp. capripneumoniae Strains, Based on Polymorphisms in the 16S rRNA Genes J. Bacteriol., May 1, 1998; 180(9): 2350 - 2358. [Abstract] [Full Text]
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