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Spiroplasma cantharicola sp. nov., from Cantharid Beetles (Coleoptera: Cantharidae)

¹Insect Biocontrol Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, U. S. Department of Agriculture, Beltsville, Maryland 20705
²FacultÉ de MÉdecine, 29285 Brest CÉdex France
⁵Laboratoire de Biologie Cellulaire et MolÉculaire, Institut National de la Recherche Agronomique, 33883 Villenave d'Ornon CÉdex France
³Department of Biology, Tuskegee University, Tuskegee, Alabama 36088
⁴Mycoplasma Section, Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Frederick Cancer Research Facility, Frederick, Maryland 21702
⁶Department of Anatomical Sciences, State University of New York at Stony Brook, Stony Brook, New York 11794

^* Corresponding author.

ABSTRACT

Spiroplasma strain CC-1^T, isolated from the gut of the soldier beetle Cantharis carolinus, was serologically distinct from other spiroplasma species, groups, and subgroups. Cells of strain CC-1^T were shown by light microscopy to be helical, motile filaments. Electron microscopy showed that the cells were bounded by a single cytoplasmic membrane, with no evidence of a cell wall. The organism was insensitive to penicillin. Strain CC-1^T grew well in SM-1, M1D, and SP-4 liquid media under aerobic or anaerobic conditions. The strain also grew in 1% serum fraction medium. Optimal growth occurred at 32°C, with a doubling time of 2.6 h, but the strain multiplied at temperatures of 10 to 37°C. Strain CC-1^T produced acid from glucose but hydrolyzed neither arginine nor urea. The guanine-plus-cytosine (G+C) content of the DNA was 26± 1 mol%. Other uncloned isolates from C. carolinus exhibited similar or identical serological patterns. On the basis of the data presented here, strain CC-1^T (= ATCC 43207), previously proposed as the representative strain of subgroup XVI-1, is designated the type strain of a new species, Spiroplasma cantharicola.