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1Research Laboratory, Higeta Shoyu Co., Ltd., Choshi, Chiba 288, Japan
2Department of Agricultural Chemistry Faculty of Agriculture, Tokyo University of Agriculture, Setagaya-ku, Tokyo 156, Japan
3Department of Food Science and Technology, Faculty of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan
* Corresponding author.
ABSTRACT
Thirty-five Bacillus brevis strains obtained from culture collections, including protein-producing isolates, were taxonomically studied by using numerical analysis, DNA base composition, and DNA-DNA hybridization. Six DNA relatedness groups were represented, and these groups correlated well with clusters based on the numerical analysis. The B. brevis strains were separated into B. brevis sensu stricto, four new species, and an unidentified species of the genus Bacillus. Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov. are proposed.
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