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Use of 16S rRNA Analysis To Investigate Phylogeny of Methylotrophic Bacteria

Bonnie Jo Bratina, Gregory A. Brusseau and Richard S. Hanson^*

Gray Freshwater Biological Institute, Department of Microbiology, University of Minnesota, Navarre, Minnesota 55392

^* Corresponding author.

ABSTRACT

Small-subunit rRNAs from 24 gram-negative methylotrophic bacteria have been sequenced. A phylogenetic tree was constructed on the basis of sequence similarities by using a weighted least-mean-square difference method. The methylotrophs were separated into coherent clusters in which bacteria in each group shared physiological characteristics.

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