HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wu, C.-C. Articles by Moore, L. V. H. Search for Related Content PubMed Articles by Wu, C.-C. Articles by Moore, L. V. H. Agricola Articles by Wu, C.-C. Articles by Moore, L. V. H.

Emended Descriptions of Prevotella denticola, Prevotella loescheii, Prevotella veroralis, and Prevotella melaninogenica

¹Culture Collection and Research Center, Food Industry Research and Development Institute, Hsinchu 30099, Taiwan, Republic of China
²Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0305

^* Corresponding author.

ABSTRACT

During studies of human periodontal disease, a number of bacterial strains were encountered that, on the basis of results of standard biochemical tests, appeared to be Prevotella buccalis, Prevotella denticola, Prevotella melaninogenica, or Prevotella loescheii. However, use of the standard biochemical tests, cellular fatty acid analyses, and the polyacrylamide gel electrophoresis patterns of soluble proteins resulted in conflicting identifications of these strains. The results of tests for cellobiose fermentation, inulin fermentation, and pigment production were responsible for most of the discordant results. Cellular fatty acid analyses in which the Microbial Identification System was used did not differentiate these strains from validly described species, even though separate library entries were created for them. DNA reassociation determinations in which the S1 nuclease procedure was used showed that cellobiose fermentation and pigment production are variable among strains of P. melaninogenica and P. denticola and that fermentation of xylan is not a reliable characteristic for differentiating P. buccalis from Prevotella veroralis. In contrast to previous indications, most strains of P. veroralis do not ferment xylan. These species can be differentiated by DNA-DNA reassociation and by cellular fatty acid analysis, using the Microbial Identification System, but differentiation by currently described phenotypic characteristics is not reliable. Similarly, P. loescheii and the genetically distinct (but closely related) D1C-20 group cannot be distinguished reliably from each other or from P. veroralis, P. denticola, and P. melaninogenica on the basis of currently described phenotypic tests other than cellular fatty acid composition or, for some species, electrophoretic patterns of soluble whole-cell proteins.

This article has been cited by other articles:

				C. Alauzet, F. Mory, J.-P. Carlier, H. Marchandin, E. Jumas-Bilak, and A. Lozniewski Prevotella nanceiensis sp. nov., isolated from human clinical samples Int J Syst Evol Microbiol, October 1, 2007; 57(10): 2216 - 2220. [Abstract] [Full Text] [PDF]

				M. Sakamoto, M. Suzuki, Y. Huang, M. Umeda, I. Ishikawa, and Y. Benno Prevotella shahii sp. nov. and Prevotella salivae sp. nov., isolated from the human oral cavity Int J Syst Evol Microbiol, May 1, 2004; 54(3): 877 - 883. [Abstract] [Full Text] [PDF]

				R. Gmur and T. Thurnheer Direct quantitative differentiation between Prevotella intermedia and Prevotella nigrescens in clinical specimens Microbiology, May 1, 2002; 148(5): 1379 - 1387. [Abstract] [Full Text] [PDF]