HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Touzel, J. P. Articles by Lysenko, A. M. Search for Related Content PubMed Articles by Touzel, J. P. Articles by Lysenko, A. M. Agricola Articles by Touzel, J. P. Articles by Lysenko, A. M.

DNA Relatedness among Some Thermophilic Members of the Genus Methanobacterium: Emendation of the Species Methanobacterium thermoautotrophicum and Rejection of Methanobacterium thermoformicicum as a Synonym of Methanobacterium thermoautotrophicum

¹Institut National de la Recherche Agronomique, Station de Technologie Alimentaire, B.P. 39, F-59651 Villeneuve d'Ascq Cedex, France
²Wadsworth Center for Laboratories and Research, New York State, Department of Health, and Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, New York 12201-0509
³Bacterial Genetics Group, Department of Microbiology, Agricultural University of Wageningen, NL-6703 CT Wageningen, The Netherlands
⁴Institute of Microbiology, Russian Academy of Sciences, Prospect 60-Let Oktyabrya, 7/2, Moscow 117811, Russia

^* Corresponding author.

ABSTRACT

DNA reassociation was used to determine levels of relatedness among four thermophilic Methanobacterium strains that are able to use formate and between these organisms and two representative strains of Methanobacterium thermoautotrophicum, strain H^T (= DSM 1053^T = ATCC 29096^T) (T = type strain) and strain Marburg (= DSM 2133). Three homology groups were delineated, and these groups coincided with the clusters identified by antigenic fingerprinting. The first group, which had levels of cross hybridization that ranged from 73 to 99%, included M. thermoautotrophicum H^T, Methanobacterium thermoformicicum Z-245, Methanobacterium sp. strain THF, and Methanobacterium sp. strain FTF. The second and third groups were each represented by only one strain, Methanobacterium sp. strain CB-12 and M. thermoautotrophicum Marburg, respectively (cross-hybridization levels, 13 to 30 and 29 to 33%, respectively). Our results indicate that the name M. thermoformicicum should be rejected as it is a synonym of M. thermoautotrophicum. The taxonomic positions of strains Marburg and CB-12 need further investigation.