HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Martinetti Lucchini, G. Articles by Altwegg, M. Search for Related Content PubMed Articles by Martinetti Lucchini, G. Articles by Altwegg, M. Agricola Articles by Martinetti Lucchini, G. Articles by Altwegg, M.

rRNA Gene Restriction Patterns as Taxonomic Tools for the Genus Aeromonas

Institute of Medical Microbiology, University of Zürich, Gloriastrasse 32, 8028 Zürich, Switzerland

^* Corresponding author.

ABSTRACT

In the genus Aeromonas there are at least 13 DNA hybridization groups, which are difficult to differentiate biochemically. We investigated the usefulness of rRNA gene restriction patterns for characterization and identification of the various groups. Genomic DNA was digested with restriction endonuclease SmaI, transferred to a nylon membrane, and hybridized with biotinylated plasmid pKK3535 containing the rrnB operon of Escherichia coli. The SmaI bands at 0.8 to 4 kb but not those at positions corresponding to sizes larger than 4 kb showed a good correlation with hybridization groups, allowing identification of strains to the level of genetic species. We demonstrated that the 567-bp fragment localized between positions 80 and 647 of the 16S ribosomal gene of E. coli was essential for hybridization to the low-molecular-weight fragments, whereas the remainder of the operon did not hybridize to these fragments. On the basis of these results, we concluded that the Aeromonas chromosome contains multiple rRNA operons which may be used for species identification.

This article has been cited by other articles:

				C. Esteve, L. Valera, C. Gutierrez, and A. Ventosa Taxonomic study of sucrose-positive Aeromonas jandaei-like isolates from faeces, water and eels: emendation of A. jandaei Carnahan et al. 1992 Int J Syst Evol Microbiol, September 1, 2003; 53(5): 1411 - 1419. [Abstract] [Full Text] [PDF]

				S. M. Kirov, B. C. Tassell, A. B. T. Semmler, L. A. O'Donovan, A. A. Rabaan, and J. G. Shaw Lateral Flagella and Swarming Motility in Aeromonas Species J. Bacteriol., January 15, 2002; 184(2): 547 - 555. [Abstract] [Full Text] [PDF]

				S. M. Kirov, L. A. O'Donovan, and K. Sanderson Functional Characterization of Type IV Pili Expressed on Diarrhea-Associated Isolates of Aeromonas species Infect. Immun., October 1, 1999; 67(10): 5447 - 5454. [Abstract] [Full Text] [PDF]

				W.-C. Ko, H.-M. Wu, T.-C. Chang, J.-J. Yan, and J.-J. Wu Inducible beta -Lactam Resistance in Aeromonas hydrophila: Therapeutic Challenge for Antimicrobial Therapy J. Clin. Microbiol., November 1, 1998; 36(11): 3188 - 3192. [Abstract] [Full Text]