IJSEM Journal of Clinical Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Int J Syst Bacteriol 42 (1992), 370-377; DOI 10.1099/00207713-42-3-370
© 1992 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Welsh, J.
Right arrow Articles by Mcclelland, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Welsh, J.
Right arrow Articles by Mcclelland, M.
Agricola
Right arrow Articles by Welsh, J.
Right arrow Articles by Mcclelland, M.

Genomic Fingerprinting by Arbitrarily Primed Polymerase Chain Reaction Resolves Borrelia burgdorferi into Three Distinct Phyletic Groups

John Welsh1,*, Charles Pretzman2, Danielle Postic3, Isabelle Saint Girons3, Guy Baranton3 and Michael Mcclelland1

1California Institute of Biological Research, 11099 North Torrey Pines Road, La Jolla, California 92037
2Vector Borne Disease Unit, Ohio Department of Health, Columbus, Ohio 43201
3Unite de Bacteriologie Moleculaire et Medicale, Institut Pasteur, 75724 Paris Cedex 15, France

* Corresponding author.

ABSTRACT

The causative agent of Lyme disease, Borrelia burgdorferi, was first identified by Burgdorfer et al. in 1982 (W. Burgdorfer, A. G. Barbour, S. F. Hayes, J. L. Benach, E. Grunwaldt, and J. P. Davis, Science 216:1317-1319,1982) and was isolated by Barbour et al. in 1983 (A. G. Burbour, W. Burgdorfer, S. E. Hayes, O. Peter, and A. Aeschlimann, Curr. Microbiol. 8:123-126, 1983). Since then, a large number of isolates have been collected, and there have been questions regarding the relationships among the various strains. Using genomic fingerprinting by an arbitrarily primed polymerase chain reaction, we resolved into three groups a collection of Eurasian and North American isolates of spirochetes that are generally categorized as B. burgdorferi. Group I strains have been identified in both North America and Eurasia, while strains belonging to Borrelia groups II and III have been found only in Eurasia. These same three groups have also been delineated by Baranton et al. (G. Baranton, D. Postic, I. Saint Girons, P. Boerlin, J.-C. Piffaretti, M. Assous, and P. A. D. Grimont, Int. J. Syst. Bacteriol. 42:370-375, 1992) by independent methods. Two isolates are distinct from all of the other strains in our collection but are clearly members of the genus Borrelia.




This article has been cited by other articles:


Home page
 Int. J. Syst. Evol. Microbiol.Home page
D. Richter, D. Postic, N. Sertour, I. Livey, F.-R. Matuschka, and G. Baranton
Delineation of Borrelia burgdorferi sensu lato species by multilocus sequence analysis and confirmation of the delineation of Borrelia spielmanii sp. nov.
Int J Syst Evol Microbiol, April 1, 2006; 56(Pt 4): 873 - 881.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
Y Sirivatanauksorn, V Sirivatanauksorn, N R Lemoine, R C N Williamson, and B R Davidson
Genomic homogeneity in fibrolamellar carcinomas
Gut, July 1, 2001; 49(1): 82 - 86.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
L. Ciceroni, S. Ciarrochi, A. Ciervo, V. Mondarini, F. Guzzo, G. Caruso, R. Murgia, and M. Cinco
Isolation and Characterization of Borrelia burgdorferi Sensu Lato Strains in an Area of Italy Where Lyme Borreliosis Is Endemic
J. Clin. Microbiol., June 1, 2001; 39(6): 2254 - 2260.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
K. Ornstein, J. Berglund, I. Nilsson, R. Norrby, and S. Bergström
Characterization of Lyme Borreliosis Isolates from Patients with Erythema Migrans and Neuroborreliosis in Southern Sweden
J. Clin. Microbiol., April 1, 2001; 39(4): 1294 - 1298.
[Abstract] [Full Text]

Home page
 CVIHome page
A. G. Bretz, K. Ryffel, P. Hutter, E. Dayer, and O. Peter
Specificities and Sensitivities of Four Monoclonal Antibodies for Typing of Borrelia burgdorferi Sensu Lato Isolates
Clin. Vaccine Immunol., March 1, 2001; 8(2): 376 - 384.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
J. Pietilä, Q. He, J. Oksi, and M. K. Viljanen
Rapid Differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi Sensu Stricto by LightCycler Fluorescence Melting Curve Analysis of a PCR Product of the recA Gene
J. Clin. Microbiol., July 1, 2000; 38(7): 2756 - 2759.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
B. Jaulhac, R. Heller, F. X. Limbach, Y. Hansmann, D. Lipsker, H. Monteil, J. Sibilia, and Y. Piémont
Direct Molecular Typing of Borrelia burgdorferi Sensu Lato Species in Synovial Samples from Patients with Lyme Arthritis
J. Clin. Microbiol., May 1, 2000; 38(5): 1895 - 1900.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
M. J. Caimano, X. Yang, T. G. Popova, M. L. Clawson, D. R. Akins, M. V. Norgard, and J. D. Radolf
Molecular and Evolutionary Characterization of the cp32/18 Family of Supercoiled Plasmids in Borrelia burgdorferi 297
Infect. Immun., March 1, 2000; 68(3): 1574 - 1586.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
G. Wang, A. P. van Dam, I. Schwartz, and J. Dankert
Molecular Typing of Borrelia burgdorferi Sensu Lato: Taxonomic, Epidemiological, and Clinical Implications
Clin. Microbiol. Rev., October 1, 1999; 12(4): 633 - 653.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
D. Postic, N. M. Ras, R. S. Lane, P.-F. Humair, M. M. Wittenbrink, and G. Baranton
Common Ancestry of Borrelia burgdorferi Sensu Lato Strains from North America and Europe
J. Clin. Microbiol., September 1, 1999; 37(9): 3010 - 3012.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
D. Liveris, S. Varde, R. Iyer, S. Koenig, S. Bittker, D. Cooper, D. McKenna, J. Nowakowski, R. B. Nadelman, G. P. Wormser, et al.
Genetic Diversity of Borrelia burgdorferi in Lyme Disease Patients as Determined by Culture versus Direct PCR with Clinical Specimens
J. Clin. Microbiol., March 1, 1999; 37(3): 565 - 569.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
C. Goarant, F. Merien, F. Berthe, I. Mermoud, and P. Perolat
Arbitrarily Primed PCR To Type Vibrio spp. Pathogenic for Shrimp
Appl. Envir. Microbiol., March 1, 1999; 65(3): 1145 - 1151.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
M.-C. Misonne, G. Van Impe, and P. P. Hoet
Genetic Heterogeneity of Borrelia burgdorferi Sensu Lato in Ixodes ricinus Ticks Collected in Belgium
J. Clin. Microbiol., November 1, 1998; 36(11): 3352 - 3354.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
G. Wang, A. P. van Dam, L. Spanjaard, and J. Dankert
Molecular Typing of Borrelia burgdorferi Sensu Lato by Randomly Amplified Polymorphic DNA Fingerprinting Analysis
J. Clin. Microbiol., March 1, 1998; 36(3): 768 - 776.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
J. A. Carlyon, C. Lavoie, S.-Y. Sung, and R. T. Marconi
Analysis of the Organization of Multicopy Linear- and Circular-Plasmid-Carried Open Reading Frames in Borrelia burgdorferi Sensu Lato Isolates
Infect. Immun., March 1, 1998; 66(3): 1149 - 1158.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
F. C. Fang, M. McClelland, D. G. Guiney, M. M. Jackson, A. I. Hartstein, V. H. Morthland, C. E. Davis, D. C. McPherson, and J. Welsh
Value of Molecular Epidemiologic Analysis in a Nosocomial Methicillin-Resistant Staphylococcus aureus Outbreak
JAMA, September 15, 1993; 270(11): 1323 - 1328.
[Abstract] [PDF]

Home page
 JAMAHome page
J. R. Lupski
Molecular Epidemiology and Its Clinical Application
JAMA, September 15, 1993; 270(11): 1363 - 1364.
[Abstract] [PDF]

Home page
 ScienceHome page
A. Barbour and D Fish
The biological and social phenomenon of Lyme disease
Science, June 11, 1993; 260(5114): 1610 - 1616.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1992 by the International Union of Microbiological Societies.