HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Busse, H.-J. Articles by Auling, G. Search for Related Content PubMed Articles by Busse, H.-J. Articles by Auling, G. Agricola Articles by Busse, H.-J. Articles by Auling, G.

Identification of Xenobiotic-Degrading Isolates from the Beta Subclass of the Proteobacteria by a Polyphasic Approach Including 16S rRNA Partial Sequencing

Hans-Jürgen Busse, Tarek El-Banna, Hiroshi Oyaizu and Georg Auling^*

Institut fÜr Mikrobiologie der Universität, D-3000 Hannover I, Germany

^* Corresponding author.

ABSTRACT

Nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. The presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone Q-8) allowed allocation of these organisms to the beta subclass of the Proteobacteria. On the basis of the results of additional characterization experiments (i.e., API 20NE tests, determinations of soluble protein patterns, and DNA-DNA hybridization experiments), we classified six isolates as either Comamonas testosteroni, Comamonas acidovorans, or Alcaligenes xylosoxidans subsp. denitrificans. By using the same criteria we allocated two additional isolates to the genus Alcaligenes. A comparison of a 16S rRNA fragment (positions 1220 to 1377; Escherichia coli nomenclature) indicated that the remaining isolates should be allocated as follows: One is a member of C. testosteroni and one is a member of Acidovorax facilis, as confirmed by the results of additional DNA-DNA hybridizations; two others probably belong to the family Alcaligenaceae; six are related to "Alcaligenes eutrophus"; and one, strain NRRL 12228, occupies an isolated position.

Present address: Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Tanta, Tanta, Egypt.

Present address: Institut fÜr Mikrobiologie und Genetik, Universität Wien, A-1090 Vienna, Austria.

This article has been cited by other articles:

				A. Gohler, G. Xiong, S. Paulsen, G. Trentmann, and E. Maser Testosterone-inducible Regulator Is a Kinase That Drives Steroid Sensing and Metabolism in Comamonas testosteroni J. Biol. Chem., June 20, 2008; 283(25): 17380 - 17390. [Abstract] [Full Text] [PDF]

				A. M. Spain, A. D. Peacock, J. D. Istok, M. S. Elshahed, F. Z. Najar, B. A. Roe, D. C. White, and L. R. Krumholz Identification and Isolation of a Castellaniella Species Important during Biostimulation of an Acidic Nitrate- and Uranium-Contaminated Aquifer Appl. Envir. Microbiol., August 1, 2007; 73(15): 4892 - 4904. [Abstract] [Full Text] [PDF]

				C. Yun, D. Amakata, Y. Matsuo, H. Matsuda, and M. Kawamukai New Chitosan-Degrading Strains That Produce Chitosanases Similar to ChoA of Mitsuaria chitosanitabida Appl. Envir. Microbiol., September 1, 2005; 71(9): 5138 - 5144. [Abstract] [Full Text] [PDF]

				A. Stolz, S. Burger, A. Kuhm, P. Kampfer, and H.-J. Busse Pusillimonas noertemannii gen. nov., sp. nov., a new member of the family Alcaligenaceae that degrades substituted salicylates Int J Syst Evol Microbiol, May 1, 2005; 55(3): 1077 - 1081. [Abstract] [Full Text] [PDF]

				T. Tralau, A. M. Cook, and J. Ruff Map of the IncP1{beta} Plasmid pTSA Encoding the Widespread Genes (tsa) for p-Toluenesulfonate Degradation in Comamonas testosteroni T-2 Appl. Envir. Microbiol., April 1, 2001; 67(4): 1508 - 1516. [Abstract] [Full Text]