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Int J Syst Bacteriol 42 (1992), 151-155; DOI 10.1099/00207713-42-1-151
© 1992 Society for General Microbiology
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Polyacrylamide Gel Electrophoresis Analysis of Ribosomal Protein AT-L30 from an Actinomycete Genus, Streptosporangium

Kozo Ochi1,* and Shinji Miyadoh2

1National Food Research Institute, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan
2Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., Morooka, Kohoku-ku, Yokohama 222, Japan

* Corresponding author.

ABSTRACT

We analyzed the ribosomal AT-L30 proteins from 11 type strains of species belonging to the genus Streptosporangium. The electrophoretic mobilities of the AT-L30 preparations from these strains, as determined by two-dimensional polyacrylamide gel electrophoresis, revealed that they could be divided into three groups. The first group contained Streptosporangium viridogriseum, S. viridogriseum subsp. kofuense, and S. albidum, while the second group contained S. roseum, S. album, S. vulgare, S. nondiastaticum, S. fragile, S. violaceochromogenes, and S. amethystogenes. S. corrugatum was a member of the third group. These groups were completely consistent with Nonomura's previous classification, which was based on morphological criteria. The results of partial amino acid sequencing of AT-L30 preparations from several representative strains strongly supported the hypothesis that each of the three groups of the genus Streptosporangium merits separate generic status.







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Copyright © 1992 by the International Union of Microbiological Societies.