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Int J Syst Bacteriol 41 (1991), 343-346; DOI 10.1099/00207713-41-3-343
© 1991 Society for General Microbiology
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Comparative Analysis of Bacillus anthracis, Bacillus cereus, and Related Species on the Basis of Reverse Transcriptase Sequencing of 16S rRNA

Carol Ash1, John A. E. Farrow1, Matthias Dorsch2, Erko Stackebrandt2 and Matthew D. Collins1,*

1Department of Microbiology, AFRC Institute of Food Research, Shinfield, Reading, RG2 9AT, United Kingdom
2Department of Microbiology, University of Queensland, Queensland 4072, Australia

* Corresponding author.

ABSTRACT

The primary structures of the 16S rRNAs of Bacillus anthracis, Bacillus cereus, Bacillus mycoides, and Bacillus thuringiensis were determined by using the reverse transcription-dideoxy sequencing method. All of the strains exhibited very high levels of sequence similarity T (T = type strain) by only a single nucleotide. The 16S rRNA sequences of B. mycoides and B. thuringiensis differed from each other and from the sequences of B. anthracis and B. cereus by four to nine nucleotides.




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