HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by BOERLIN, P. Articles by PIFFARETTI, J.-C. Search for Related Content PubMed PubMed Citation Articles by BOERLIN, P. Articles by PIFFARETTI, J.-C. Agricola Articles by BOERLIN, P. Articles by PIFFARETTI, J.-C.

Taxonomy of the Genus Listeria by Using Multilocus Enzyme Electrophoresis

¹Istituto Cantonale Batteriologico, Via Ospedale 6, 6904 Lugano, Switzerland
²UnitÉ d'Ecologie BactÉrienne, Centre National de RÉfÉrence pour la Lysotypie et le Typage MolÉculaire de Listeria, Institut Pasteur, 75724 Paris Cedex 15, France

^* Corresponding author.

ABSTRACT

Seventy-three strains of the seven recognized Listeria species were studied by performing a multilocus enzyme electrophoresis analysis of 18 enzyme loci. The mean number of alleles per locus was 9.5 and all of the loci were polymorphic. A total of 56 electrophoretic types were distinguished. Cluster analysis of a matrix of the genetic distances between paired electrophoretic types revealed that there were six principal clusters at the species level (genetic distances between clusters greater than 0.8). Listeria monocytogenes, Listeria innocua, Listeria welshimeri, Listeria seeligeri, and Listeria ivanovii each corresponded to one of these clusters with no overlap. Our results are in agreement with those of previous DNA hybridization experiments (Rocourt et al., Curr. Microbiol. 7:383-388, 1982). Listeria grayi and Listeria murrayi electrophoretic types formed a unique cluster, thus reinforcing the suggestion of Wilkinson and Jones (J. Gen. Microbiol. 98:399-421, 1977) that these two species should be considered two biovars of a single species.

This article has been cited by other articles:

				D. Paillard, V. Dubois, R. Duran, F. Nathier, C. Guittet, P. Caumette, and C. Quentin Rapid Identification of Listeria Species by Using Restriction Fragment Length Polymorphism of PCR-Amplified 23S rRNA Gene Fragments Appl. Envir. Microbiol., November 1, 2003; 69(11): 6386 - 6392. [Abstract] [Full Text] [PDF]

				P. Gilot and J. Content Specific Identification of Listeria welshimeri and Listeria monocytogenes by PCR Assays Targeting a Gene Encoding a Fibronectin-Binding Protein J. Clin. Microbiol., February 1, 2002; 40(2): 698 - 703. [Abstract] [Full Text] [PDF]

				M. Gutacker, C. Valsangiacomo, and J.-C. Piffaretti Identification of two genetic groups in Bacteroides fragilis by multilocus enzyme electrophoresis: distribution of antibiotic resistance (cfiA, cepA) and enterotoxin (bft) encoding genes Microbiology, May 1, 2000; 146(5): 1241 - 1254. [Abstract] [Full Text]

				P. Gilot, M. Gubina, N. Klun, J. Mehle, E. Tcherneva, N. Rijpens, and L. Herman Typing of Listeria monocytogenes Strains by Repetitive Element Sequence-Based PCR J. Clin. Microbiol., January 1, 1999; 37(1): 103 - 109. [Abstract] [Full Text]