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DNA Relatedness among Strains of Leptospira biflexa

¹Department of Veterinary Pathology and Public Health, Faculty of Veterinary Science New Zealand, and Instituto Di Microbiologia, Universita Degli Studi De Trieste, 34127 Trieste, Italy
²Department of Microbiology and Genetics, New Zealand, and Instituto Di Microbiologia, Universita Degli Studi De Trieste, 34127 Trieste, Italy
³Massey University, Palmerston North, New Zealand, and Instituto Di Microbiologia, Universita Degli Studi De Trieste, 34127 Trieste, Italy

^* Corresponding author.

ABSTRACT

The slot blot method of DNA hybridization was used to study 38 strains of Leptospira biflexa belonging to 38 serovars. Fifteen of these serovars were placed into six groups. The remaining 23 serovars were generally too diverse to show significant DNA relatedness either to these groups or to one another. Serovar thracia was related to Group 5, but it was not included in this group because its percent relatedness was too low. We found that genetically related organisms were antigenically dissimilar. The absence of any significant genetic relationship between Leptonema illini and the Leptospira biflexa serovars tested supports the placement of the former species in a separate genus.

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