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Description of Bacillus naganoensis sp. nov.

Enzyme Bio-Systems Ltd., 3350 Salt Creek Lane, Arlington Heights, Illinois 60005

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ABSTRACT

A new species, Bacillus naganoensis, is proposed for an obligately aerobic, moderately acidophilic, endospore-forming bacterium that produces a thermostable, aciduric pullulanase (EC 3.2.1.41). The organism was isolated from soil by selection on solid, pullulan-containing medium at pH 4.0 and 30°C. The isolate required a medium pH of less than 6.5 for growth initiation. Fatty acid composition studies revealed that the major fatty acid of cells grown in nutrient broth supplemented with 1% starch was 14-methylpentadecanoic acid (iso-C₁₆) at 45 mol%. The guanine-plus-cytosine content of the DNA of this organism was 45 ± 2 mol%. A type culture has been deposited with the American Type Culture Collection, Rockville, Md., as strain ATCC 53909.

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