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Int J Syst Bacteriol 31 (1981), 35-42; DOI 10.1099/00207713-31-1-35
© 1981 Society for General Microbiology
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Deoxyribonucleic Acid Relatedness Between Enterobacter cloacae and Enterobacter amnigenus sp. nov.

D. IZARD, F. GAVINI, P. A. TRINEL and H. LECLERC

Institut Pasteur de Lille, Unité INSERM 146, Université de Lille II, Domaine du C.E.R.T.I.A., 59651 Villeneuve d'Ascq Cedex, France

ABSTRACT

A deoxyribonucleic acid (DNA)-DNA hybridization study was carried out to determine the taxonomic position of a new group of enterobacters (group H3, Gavini et al. [Ann. Microbiol. (Inst. Pasteur) 127B:317-335, 1976]) previously studied by numerical taxonomy. The reassociation binding ratios with the group H3 strain ATCC 33072 ranged from 61 to 97%. With respect to DNA hybridization, this group, which represents a new species in the genus Enterobacter, Enterobacter amnigenus, is closely related (49 to 66%) to Enterobacter cloacae. A difference in genome size between E. amnigenus ATCC 33072 and E. cloacae Collection de l'Unité d'Écotoxicologie Microbienne (CUETM) 77-120 was observed after reciprocal binding: The genome of ATCC 33072 was 24% larger. E. amnigenus was defined from phenotypic and genetic data. The type strain of E. amnigenus is CUETM 77-118 (ATCC 33072).




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